Biodecolorization of Textile Dyes by Immobilized Enzymes in a Vertical Bioreactor System
Biodecolorization of Textile Dyes by Immobilized Enzymes in a Vertical Bioreactor System
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立式生物反应器系统中固定化酶对纺织染料的生物脱色
DOI：
10.1016/j.proenv.2014.03.030
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
K. Itoh
中科院分区：
文献类型：
--
作者：
D. Yanto;S. Tachibana;K. Itoh
来自 Trametes versicolorU97 或 Pestalotiopsissp 的固定化酶的能力。在立式生物反应器系统中研究了 NG007 对三种纺织染料的脱色作用。使用双层藻酸盐珠（1.5% w/w）和粗酶进行固定。研究了介质：吐温80、1-羟基苯并三唑(HBT)或2,2,6,6-四甲基哌啶-1-基)氧基(TEMPO)对脱色率的影响。结果表明，在立式生物反应器中应用固定化酶提高染料脱色效率的顺序分别为LB16>RRV9>RRN4。 U97固定化酶中使用介体TEMPO或NG007中使用Tween 80时，脱色速度加快。与戊二醛 (0.6%) 反应 4 小时，保持了珠子的寿命和可重复使用性。该研究表明，生物反应器系统中的双层固定化酶对于处理纺织染料废水具有巨大的潜在策略。
A capacity of immobilized enzymes fromTrametes versicolorU97 orPestalotiopsissp. NG007 to decolorize three textile dyes was investigated in a vertical bioreactor system. Immobilization was conducted using a double layer of alginate bead (1.5% w/w) and crude enzymes. The effect of mediators: Tween 80, 1-hydroxybenzotriazol (HBT), or 2,2,6,6-tetramethylpiperidin-1-yl)oxyl (TEMPO) was investigated on decolorization rate. The results showed that application of immobilized enzymes in a vertical bioreactor enhanced the decolorization efficiency of dyes in the order LB16>RRV9>RRN4, respectively. The decolorization rate was accelerated when a mediator TEMPO was used in immobilized enzyme of U97 or a Tween 80 was used in NG007. Reaction with glutaraldehyde (0.6%) for 4 h maintained the longevity and reusability of the beads. The study suggests that double layer immobilized enzyme in a bioreactor system has a terrific potential strategy for treating the textile dye effluents.