Proton flux through the chloroplast ATP synthase is altered by cleavage of its gamma subunit.
Proton flux through the chloroplast ATP synthase is altered by cleavage of its gamma subunit.
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通过叶绿体 ATP 合酶的质子通量因其 γ 亚基的裂解而改变。
DOI：
10.1016/j.bbabio.2007.04.008
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
R. E. Mccarty
中科院分区：
文献类型：
--
作者：
J. R. McCallum;R. E. Mccarty
电子传递，质子梯度和ATP的合成测定类囊体已被短暂暴露于低浓度的胰蛋白酶在照明。这种处理将ATP合酶的γ亚基切割成两个大片段，它们仍然与酶结合。与对照膜相比，胰蛋白酶处理的膜需要更高的电子传输速率才能产生给定的质子梯度值，这表明处理过的膜是质子泄漏的。由于venturicidin恢复电子传递和质子梯度控制水平，质子泄漏是通过ATP合酶。值得注意的是，由胰蛋白酶处理的膜在饱和光强度的ATP的合成仅轻微抑制，即使质子梯度是显着较低的处理类囊体。ATP合成和质子梯度被确定为控制和胰蛋白酶处理的类囊体中的光强度的函数。胰蛋白酶处理的膜合成ATP在较低的值的质子梯度比控制膜。γ亚基的裂解消除了ε亚基对叶绿体ATP合酶活性的抑制。我们的研究结果表明，克服抑制ε亚基成本的能量。
Electron transport, the proton gradient and ATP synthesis were determined in thylakoids that had been briefly exposed to a low concentration of trypsin during illumination. This treatment cleaves the γ subunit of the ATP synthase into two large fragments that remain associated with the enzyme. Higher rates of electron transport are required to generate a given value of the proton gradient in the trypsin-treated membranes than in control membranes, indicating that the treated membranes are proton leaky. Since venturicidin restores electron transport and the proton gradient to control levels, the proton leak is through the ATP synthase. Remarkably, the synthesis of ATP by the trypsin-treated membranes at saturating light intensities is only slightly inhibited even though the proton gradient is significantly lower in the treated thylakoids. ATP synthesis and the proton gradient were determined as a function of light intensity in control and trypsin-treated thylakoids. The trypsin-treated membranes synthesized ATP at lower values of the proton gradient than the control membranes. Cleavage of the γ subunit abrogates inhibition of the activity of the chloroplast ATP synthase by the ε subunit. Our results suggest that overcoming inhibition by the ε subunit costs energy.
DOI：
10.1016/s0021-9258(17)39870-8
发表时间：
1984-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Stuart R. Ketcham;James W. Davenport;Kurt WarnckelI;R. E. Mccarty
通讯作者：
Stuart R. Ketcham;James W. Davenport;Kurt WarnckelI;R. E. Mccarty
制备重组活性形式的ε亚基和ε亚基缺陷型叶绿体耦合因子1。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Richter,ML;Patrie,WJ;McCarty,RE
通讯作者：
McCarty,RE
胰蛋白酶对耦合因子 1 的 γ 亚基进行光依赖性裂解，导致 Mg2-ATP 酶活性激活，并解偶联菠菜叶绿体中的光磷酸化。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Moroney,JV;McCarty,RE
通讯作者：
McCarty,RE