A novel mammalian HORMA domain-containing protein, HORMAD1, preferentially associates with unsynapsed meiotic chromosomes
A novel mammalian HORMA domain-containing protein, HORMAD1, preferentially associates with unsynapsed meiotic chromosomes
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DOI：
10.1016/j.yexcr.2009.08.007
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发表时间：
2010-01-15
影响因子：
3.7
通讯作者：
Hoog, Christer
中科院分区：
文献类型：
--
作者：
Fukuda, Tomoyuki;Daniel, Katrin;Hoog, Christer
含有 HORMA 结构域的蛋白质在多种真核生物减数分裂过程中调节同源染色体（同源物）之间的相互作用。我们已经鉴定出一种含有小鼠 HORMA 结构域的蛋白质 HORMAD1，并通过生化和细胞学研究表明它与减数分裂染色体轴相关。 HORMAD1 首先在减数分裂前期 I 的细线期到合子期期间在染色体上积累。随着生殖细胞进入粗线期，HORMAD1 从突触染色体区域消失。然而，一旦染色体在双线期期间去突触，HORMAD1 就会再次积聚在去突触同源物的染色体轴上。因此，在减数分裂前期 I 阶段，HORMAD1 优先定位于未突触或去突触的染色体区域。对缺乏联会复合体（SC）不同成分的突变株的分析表明，联会复合体的建立是 HORMAD1 从染色体轴上移位所必需的。因此，我们的结果强烈表明哺乳动物细胞也使用含有 HORMA 结构域的蛋白质作为监视系统的一部分，该监视系统监视突触或同源物之间的其他相互作用。 (C) 2009 Elsevier Inc. 保留所有权利。
HORMA domain-containing proteins regulate interactions between homologous chromosomes (homologs) during meiosis in a wide range of eukaryotes. We have identified a mouse HORMA domain-containing protein, HORMAD1, and biochemically and cytologically shown it to be associated with the meiotic chromosome axis. HORMAD1 first accumulates on the chromosomes during the leptotene to zygotene stages of meiotic prophase I. As germ cells progress into the pachytene stage, HORMAD1 disappears from the synapsed chromosomal regions. However, once the chromosomes desynapse during the diplotene stage, HORMAD1 again accumulates on the chromosome axis of the desynapsed homologs. HORMAD1 thus preferentially localizes to unsynapsed or desynapsed chromosomal regions during the prophase I stage of meiosis. Analysis of mutant strains lacking different components of the synaptonemal complex (SC) revealed that establishment of the SC is required for the displacement of HORMAD1 from the chromosome axis. Our results therefore strongly suggest that also mammalian cells use a HORMA domain-containing protein as part of a surveillance system that monitors synapsis or other interactions between homologs. (C) 2009 Elsevier Inc. All rights reserved.