Strand-Specific RNA-Seq Applied to Malaria Samples.
Strand-Specific RNA-Seq Applied to Malaria Samples.
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链特异性 RNA 测序应用于疟疾样本。
DOI：
10.1007/978-1-0716-0743-5_2
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
LeRoch,Karine
中科院分区：
文献类型：
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作者：
Lu,XueqingMaggie;LeRoch,Karine
仅在过去的几年里，下一代测序技术就变得可用，许多应用程序也迅速衍生出来，例如rna-seq的开发，这是一种使用深度测序来描绘整个转录本的技术。RNA-seq有能力发现新的转录本和剪接变异体、单核苷酸变异、融合基因和基于mRNA水平的表达谱。准备RNA-seq文库可能很精细，通常需要购买昂贵的试剂盒，需要大量的陈述材料。本文提出的方法是灵活的，具有成本效益。利用这种方法，我们从人疟疾寄生虫恶性疟原虫中提取的RNA制备了高质量的链特异性RNAseq文库，该文库与Illumina®的S测序仪和Hi-Seq兼容。然而，该方法可以很容易地适用于其他平台。
Over the past few years only, next-generation sequencing technologies became accessible and many applications were rapidly derived, such as the development of RNA-seq, a technique that uses deep sequencing to profile whole transcriptomes. RNA-seq has the power to discover new transcripts and splicing variants, single nucleotide variations, fusion genes, and mRNA levels-based expression profiles. Preparing RNA-seq libraries can be delicate and usually obligates buying expensive kits that require large amounts of stating materials. The method presented here is flexible and cost-effective. Using this method, we prepared high-quality strand-specific RNA-seq libraries from RNA extracted from the human malaria parasitePlasmodium falciparum.The libraries are compatible with Illumina®’s sequencers Genome Analyzer and Hi-Seq. The method can however be easily adapted to other platforms.