Congenital deficiency of fibrin-stabilizing factor (factor XIII). A report of the first case from Latin America.
Congenital deficiency of fibrin-stabilizing factor (factor XIII). A report of the first case from Latin America.
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先天性纤维蛋白稳定因子（因子 XIII）缺乏。
DOI：
10.1159/000208923
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发表时间：
1968
影响因子：
2.4
通讯作者：
J. R. Rangel
中科院分区：
文献类型：
--
作者：
T. Arends;J. M. Guevara;J. R. Rangel
方法凝血试验按照标准方法[15]进行，血浆凝块在5M尿素中的溶解度按照LOSOWSKY等[8]进行。这些患者的缺陷在于凝块，人们认为用胰蛋白酶将其重新溶解并进行电泳研究，与正常受试者相比可能会观察到差异。使用以下方法：将0.5ml草酸血浆与0.5ml 0.025M CaCl2混合，在37℃下孵育30分钟；将凝块拍松，用0.85% NaCl洗涤3次，然后用一滴市售胰蛋白酶（NBCo）4mg/ml在37℃下胰蛋白酶消化2h，间隔轻轻摇动2或3次；用0.5M乙酸调节pH至6.4停止胰蛋白酶消化，并以2000rpm离心。将样品上清液进行醋酸纤维素电泳6次。
MethodsThe coagulation tests were performed by the standard methods [15], and the plasma clot solubility in 5M urea was done according to LOSOWSKY et al.[8]. The defect of these patients being in the clot, it was thought that re-dissolving it with trypsin and studying it clcctrophoretically, a difference might be observed when compared with the normal subjects. The following method was used: 0.5 ml of oxalatcd plasma mixed with 0.5 ml of 0.025 M of CaCl2, incubated 30 min at 37 C; the clot was tapped loose and washed 3 times with NaCl 0.85% and then trypsin digested with a drop of commercial trypsin (NBCo) 4 mg/ml at 37 C for 2 h, shaking gently at intervals 2 or 3 times; trypsin digestion was stopped adjusting to pH 6.4 with 0.5 M acetic acid, and centrifugated at 2000 rpm. Acetate cellulose electro phoresis of the supernatant liquid was made applying the sample 6 times.