Pseudoxanthomonas putridarboris sp nov isolated from rotten tree
Pseudoxanthomonas putridarboris sp nov isolated from rotten tree
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DOI：
10.1099/ijsem.0.001867
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发表时间：
2017-06-01
影响因子：
2.8
通讯作者：
Roh, Dong-Hyun
中科院分区：
文献类型：
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作者：
Lee, Je-Kwan;Oh, Ji-Sung;Roh, Dong-Hyun
从韩国忠北国立大学的一棵腐烂的树上分离出一种革兰氏染色阴性、杆状、能动的细菌，命名为 WD12(T)。 WD12(T)在30-37℃和pH 7.0-7.5下生长最佳，并且可以同化熊果苷和5-酮葡萄糖酸钾。主要细胞脂肪酸为 iso-C-16 : 0、C-16 : 0、环 C-17 : 0、iso-C-15 : 0、总结特征 3（包含 C-16 : 1 omega 7c/iso-C-15 : 0 2-OH）和 anteiso-C-15 : 0。主要极性脂质包括磷脂酰乙醇胺、二磷脂酰甘油和磷脂酰甘油。主要的呼吸醌是泛醌-8 (Q-8)。基因组DNA的G+C含量为69.1%。基于16S rRNA基因序列的系统发育和比较分析结果表明，WD12(T)与黄单胞菌科假黄单胞菌属的Pseudoxanthomonas mexicana AMX 26B(T)和Pseudoxanthomonas japonensis 12-3(T)形成了紧密的系统发育谱系。与假黄单胞菌属其他成员的序列相似性范围为 98.6% (P. mexicana AMX 26B(T)) 至 95.1% (Pseudoxanthomonas taiwanensis CB-226(T))。 WD12(T)与假黄单胞菌属物种的八种典型菌株之间的DNA-DNA相关性显示出超过97％的16S rRNA序列相似性为6+/-0-26+/-1％。根据这项多相研究的证据，WD12(T) 代表了假黄单胞菌属的一个新物种，其名称为 Pseudoxanthomonas putridarboris sp。十一月被提议。模式菌株为 WD12(T)（= KACC 15045(T) = LMG 25968(T)）。
A Gram-staining-negative, rod-shaped, motile bacterium, designated WD12(T), was isolated from a rotten tree at Chungbuk National University, South Korea. WD12(T) grew optimally at 30-37 degrees C and pH 7.0-7.5 and could assimilate arbutin and potassium-5-ketogluconate. The major cellular fatty acid were iso-C-16 : 0, C-16 : 0, cyclo C-17 : 0, iso-C-15 : 0, summed features 3 (comprising C-16 : 1 omega 7c/iso-C-15 : 0 2-OH) and anteiso-C-15 : 0. The major polar lipids consisted of phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The major respiratory quinone was ubiquinone-8 (Q-8). The G+ C content of the genomic DNA was 69.1 %. The results of phylogenetic and comparative analysis based on the 16S rRNA gene sequence indicated that WD12(T) formed a tight phylogenetic lineage with Pseudoxanthomonas mexicana AMX 26B(T) and Pseudoxanthomonas japonensis 12-3(T) of the the genus Pseudoxanthomonas in the family Xanthomonadaceae. Sequence similarity to other members of the genus Pseudoxanthomonas ranged from 98.6% (P. mexicana AMX 26B(T)) to 95.1% (Pseudoxanthomonas taiwanensis CB-226(T)). DNA-DNA relatedness between WD12(T) and eight type strains of species of the genus Pseudoxanthomonas showing more than 97% 16S rRNA sequence similarity were 6 +/- 0-26 +/- 1 %. On the basis of the evidence from this polyphasic study, WD12(T) represents a novel species of the genus Pseudoxanthomonas, for which the name Pseudoxanthomonas putridarboris sp. nov. is proposed. The type strain is WD12(T) (= KACC 15045(T) = LMG 25968(T)).