Development of andrographolide molecularly imprinted polymer for solid-phase extraction.
Development of andrographolide molecularly imprinted polymer for solid-phase extraction.
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DOI：
10.1016/j.saa.2011.02.034
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发表时间：
2011-06
期刊：
影响因子：
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通讯作者：
X. Yin;Qing-shan Liu;Y. Jiang;Yong-ming Luo
中科院分区：
文献类型：
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作者：
X. Yin;Qing-shan Liu;Y. Jiang;Yong-ming Luo
建立了一种以分子印迹聚合物（MIP）为固相萃取（SPE）选择性吸附剂的样品前处理方法。以穿心莲内酯为模板分子，采用沉淀聚合法制备了聚合物。对分子印迹聚合物的结构进行了表征，并采用Scatchard方程测定了其静态吸附量。与C18-SPE和非印迹聚合物（NIP）SPE柱相比，MIP-SPE柱对穿心莲提取物中的穿心莲内酯和去氢穿心莲内酯具有较高的选择性和较好的亲和性。Nees（APN）。MIP-SPE柱对穿心莲内酯和去氢穿心莲内酯的柱容量分别为11.9±0.6μmol/g和12.1±0.5μmol/g，是其它两种柱的2-3倍。穿心莲内酯和去氢穿心莲内酯的回收率分别为96.0%和104.2%（RSD分别为2.9%和3.7%）。采用各种真实的样品验证了方法的可行性。该方法显示了分子印迹固相萃取技术在快速、选择性和有效的样品前处理中的应用潜力。
A method employing molecularly imprinted polymer (MIP) as selective sorbent for solid-phase extraction (SPE) to pretreat samples was developed. The polymers were prepared by precipitation polymerization with andrographolide as template molecule. The structure of MIP was characterized and its static adsorption capacity was measured by the Scatchard equation. In comparison with C18-SPE and non-imprinted polymer (NIP) SPE column, MIP-SPE column displays high selectivity and good affinity for andrographolide and dehydroandrographolide for extract of herb Andrographis paniculata (Burm.f.) Nees (APN). MIP-SPE column capacity was 11.9±0.6μmol/g and 12.1±0.5μmol/g for andrographolide and dehydroandrographolide, respectively and was 2–3 times higher than that of other two columns. The precision and accuracy of the method developed were satisfactory with recoveries between 96.4% and 103.8% (RSD 3.1–4.3%, n=5) and 96.0% and 104.2% (RSD 2.9–3.7%, n=5) for andrographolide and dehydroandrographolide, respectively. Various real samples were employed to confirm the feasibility of method. This developed method demonstrates the potential of molecularly imprinted solid phase extraction for rapid, selective, and effective sample pretreatment.