Neural crest cell-placodal neuron interactions are mediated by Cadherin-7 and N-cadherin during early chick trigeminal ganglion assembly.
Neural crest cell-placodal neuron interactions are mediated by Cadherin-7 and N-cadherin during early chick trigeminal ganglion assembly.
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DOI：
10.12688/f1000research.122686.2
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Taneyhill, Lisa A
中科院分区：
文献类型：
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作者：
Halmi, Caroline A;Wu, Chyong-Yi;Taneyhill, Lisa A
背景：颅神经节位于头部的不同位置，是整合各种感觉输入的关键。其中最大的神经节是三叉神经节，它通过三个主要神经分支将疼痛、触摸和温度信息传递到中枢神经系统。三叉神经节及其神经由神经脊细胞和胎盘细胞两种重要的胚胎细胞类型的衍生物组成，它们从不同的解剖位置迁移，结合在一起，分化形成三叉神经感觉神经元和支持胶质细胞。虽然三叉神经节的双重细胞起源已经知道60多年了，但由神经脊细胞和胎盘细胞表达的调节初始神经节组装的分子仍然不清楚。以往的研究表明，细胞表面钙粘素蛋白在三叉神经节早期发生过程中具有重要作用，钙粘素-7和神经钙粘蛋白(N-cadherin)分别在神经脊细胞和胎盘细胞中表达。尽管钙粘附素通常以同嗜性(即相似)的方式相互作用，但在神经脊细胞和胎盘细胞中表达不同的钙粘附素提出了一个问题，即是否也可能发生异嗜性钙粘附素相互作用。鉴于此，本研究的目的是了解在三叉神经节形成初期，钙粘附素-7和N-钙粘附素是否相互作用。方法：为了评估钙粘附素-7和N-钙粘附素之间的潜在相互作用，我们使用了生物化学和创新的成像分析方法，包括在形成鸡的三叉神经节中进行的体外和体内实验。结果：我们的数据揭示了钙粘附素-7和N-钙粘附素之间的物理相互作用。结论：这些研究发现了一个新的分子基础，神经脊细胞和胎盘细胞可以在体内聚集，在胚胎发育过程中建立三叉神经节。
Background: Arising at distinct positions in the head, the cranial ganglia are crucial for integrating various sensory inputs. The largest of these ganglia is the trigeminal ganglion, which relays pain, touch and temperature information through its three primary nerve branches to the central nervous system. The trigeminal ganglion and its nerves are composed of derivatives of two critical embryonic cell types, neural crest cells and placode cells, that migrate from different anatomical locations, coalesce together, and differentiate to form trigeminal sensory neurons and supporting glia. While the dual cellular origin of the trigeminal ganglion has been known for over 60 years, molecules expressed by neural crest cells and placode cells that regulate initial ganglion assembly remain obscure. Prior studies revealed the importance of cell surface cadherin proteins during early trigeminal gangliogenesis, with Cadherin-7 and neural cadherin (N-cadherin) expressed in neural crest cells and placode cells, respectively. Although cadherins typically interact in a homophilic ( i.e., like) fashion, the presence of different cadherins expressed in neural crest cells and placode cells raises the question as to whether heterophilic cadherin interactions may also be occurring. Given this, the aim of the study was to understand whether Cadherin-7 and N-cadherin were interacting during initial trigeminal ganglion formation. Methods: To assess potential interactions between Cadherin-7 and N-cadherin, we used biochemistry and innovative imaging assays conducted in vitro and in vivo, including in the forming chick trigeminal ganglion. Results: Our data revealed a physical interaction between Cadherin-7 and N-cadherin. Conclusions: These studies identify a new molecular basis by which neural crest cells and placode cells can aggregate in vivo to build the trigeminal ganglion during embryogenesis.