Cloning and expression of two genes coding for sodium pumps in the salt-tolerant yeast Debaryomyces hansenii
Cloning and expression of two genes coding for sodium pumps in the salt-tolerant yeast Debaryomyces hansenii
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DOI：
10.1128/jb.183.10.3251-3255.2001
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发表时间：
2001-05-01
影响因子：
3.2
通讯作者：
Ramos, J
中科院分区：
文献类型：
--
作者：
Almagro, A;Prista, C;Ramos, J
克隆并测序了来自汉逊德巴利酵母的两个编码 Na+-ATP酶的基因。这些基因被命名为来自汉逊汉逊酵母 (DhENA1) 的 ENA1 和 DhENA2，与来自西雪旺酵母 (Schwanniomyces occidentalis) 的相应基因表现出高度同源性。 DhENA1 在高 Na+ 浓度下表达，而 DhENA2 的表达也需要高 pH 值。缺乏Na+流出系统且对Na+敏感的酿酒酵母突变体，当用DhENA1或DhENA2转化时，恢复了Na+耐受性以及挤出Na+的能力。
Two genes encoding Na+-ATPases from Debaryomyces hansenii were cloned and sequenced. The genes, designated ENA1 from D. hansenii (DhENA1) and DhENA2, exhibited high homology with the corresponding genes from Schwanniomyces occidentalis. DhENA1 was expressed in the presence of high Na+ concentrations, while the expression of DhENA2 also required high pH. A mutant of Saccharomyces cerevisiae lacking the Na+ efflux systems and sensitive to Na+, when transformed with DhENA1 or DhENA2, recovered Na+ tolerance and also the ability to extrude Na+.