GhCaM7-like, a calcium sensor gene, influences cotton fiber elongation and biomass production
GhCaM7-like, a calcium sensor gene, influences cotton fiber elongation and biomass production
复制标题
GhCaM7-like 是一种钙传感器基因，影响棉纤维伸长和生物量生产。
DOI：
10.1016/j.plaphy.2016.09.009
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发表时间：
2016-12-01
影响因子：
6.5
通讯作者：
Yang, Zuoren
中科院分区：
文献类型：
--
作者：
Cheng, Yuan;Lu, Lili;Yang, Zuoren
钙信号调节植物的许多发育过程。钙调素（CaM）是真核生物中最保守的钙离子感受器之一，具有灵活的构象。棉花是天然纤维的主要来源，其CaM的分子功能尚不清楚。本研究从高地棉中克隆了一个类似陆地棉LCaM 7的基因。生物信息学分析表明，GhCaM 7-like基因与拟南芥AtCaM 7相比高度保守。GhCaM 7-like基因在伸长纤维中表现出高表达水平。在PROGhcanv-tike：GUS融合的转基因拟南芥植物的茎、叶和根上的毛状体中观察到β-葡萄糖醛酸苷酶的表达。GhCaM 7-like基因的沉默导致纤维长度减少，但也导致茎高，叶尺寸，种子长度和百粒重减少，与对照相比。GhCaM 7-like基因表达的减少导致叶下皮层和茎尖细胞中Ca 2+内流的减少，以及GhIQD 1（含有IQ 67结构域的蛋白）、GhAnn 2（膜联蛋白）和GhEXP 2（扩展蛋白）的下调。这些结果表明GhCaM 7-like基因在钙信号通路中起着重要作用，可能通过影响Ca 2+信号和CaM下游信号通路来调控棉纤维的伸长和生物量的产生。(C)2016 Elsevier Masson SAS。All rights reserved.
Calcium signaling regulates many developmental processes in plants. Calmodulin (CaM) is one of the most conserved calcium sensors and has a flexible conformation in eukaryotes. The molecular functions of CaM are unknown in cotton, which is a major source of natural fiber. In this study, a Gossypium hirsutum LCaM7-like gene was isolated from upland cotton. Bioinformatics analysis indicated that the GhCaM7-like gene was highly conserved as compared with Arabidopsis AtCaM7. The GhCaM7-like gene showed a high expression level in elongating fibers. Expression of beta-glucuronidase was observed in trichomes on the stem, leaf and root in transgenic Arabidopsis plants of a PROGhcanv-tike:GUS fusion. Silencing of the GhCaM7-like gene resulted in decreased fiber length, but also caused reduction in stem height, leaf dimensions, seed length and 100-seed weight, in comparison with those of the control. Reduced expression of the GhCaM7-like gene caused decreased Ca2+ influx in cells of the leaf hypodermis and stem apex, and down -regulation of GhIQD1 (IQ67-domain containing protein), GhAnn2 (Annexins) and GhEXP2 (Expansin). These results indicate that the GhCaM7-like gene plays a vital role in calcium signaling pathways, and may regulate cotton fiber elongation and biomass production by affecting Ca2+ signatures and downstream signaling pathways of CaM. (C) 2016 Elsevier Masson SAS. All rights reserved.