Nonomuraea glycinis sp nov., a novel actinomycete isolated from the root of black soya bean [Glycine max (L.) Merr]
Nonomuraea glycinis sp nov., a novel actinomycete isolated from the root of black soya bean [Glycine max (L.) Merr]
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Nonomuraea giginis sp nov.，一种从黑豆根中分离出来的新型放线菌 [Glycine max (L.) Merr]
DOI：
10.1099/ijsem.0.002406
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发表时间：
2017
影响因子：
2.8
通讯作者：
Xiang Wensheng
中科院分区：
文献类型：
--
作者：
Li Zhilei;Song Wei;Zhao Junwei;Zhuang Xiaoxin;Zhao Yue;Wang Xiangjing;Xiang Wensheng
从黑豆[Glycine max（L.）从中国黑龙江省哈尔滨采集，并使用多相方法表征。该菌株是一种好氧，革兰氏染色阳性放线菌，形成广泛的分支基板菌丝和气生菌丝。主要的甲基萘醌是MK-9（H2）和MK-9（H 0）。主要细胞脂肪酸谱由异-C16：0、10-甲基C17：0和10-甲基C18：0组成。 极性脂质谱由二磷脂酰甘油、磷脂酰乙醇胺、羟基磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰肌醇甘露糖苷、磷脂酰甘油和糖脂组成。DNA G+C含量为68.2± 0.4mol%。基于16 S rRNA基因序列的系统发育分析表明，菌株NEAU-BB 2C 19 T应归属于野野村菌属，并与其最近的邻居广州野野村菌（NonomuraeaguangzhouensisNEAU-ZJ 3 T）形成明显的分支（16 S rRNA基因序列相似性为98.75%）。 形态学和化学分类学特征与Nonomuraea属一致，DNA-DNA杂交结果和一些表型特征表明菌株NEAU-BB 2C 19 T与其亲缘关系最近的菌株有明显的区别。因此，该菌株被认为代表了一个新的种属Nonomuraea，其名称为Nonomuraea glycinissp。十一是建议。模式菌株为NEAU-BB 2C 19 T（=CGMCC 4.7430T=DSM 104838 T）。
A novel actinomycete, designated strain NEAU-BB2C19T, was isolated from the root of black soya bean [Glycine max(L.) Merr] collected from Harbin, Heilongjiang Province, China, and characterized using a polyphasic approach. The strain was an aerobic, Gram-stain-positive actinomycete that formed extensively branched substrate mycelium and aerial hyphae. The predominant menaquinones were MK-9(H2) and MK-9(H0). The major cellular fatty acid profile consisted of iso-C16 : 0, 10-methyl C17 : 0and 10-methyl C18 : 0. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, phosphatidylglycerol and glycolipid. The DNA G+C content was 68.2±0.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-BB2C19Tshould be assigned to the genusNonomuraeaand formed a distinct branch with its closest neighbourNonomuraea guangzhouensisNEAU-ZJ3T(98.75 % 16S rRNA gene sequence similarity). The morphological and chemotaxonomic properties of the strain were also consistent with those of members of the genusNonomuraea .A combination of DNA–DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-BB2C19Tcould be clearly differentiated from its closest phylogenetic relative. Thus, the strain is concluded to represent a novel species of the genusNonomuraea, for which the nameNonomuraea glycinissp. nov. is proposed. The type strain is NEAU-BB2C19T(=CGMCC 4.7430T=DSM 104838T).