Direct comparison of progenitor cells derived from adipose, muscle, and bone marrow from wild-type or craniosynostotic rabbits.
Direct comparison of progenitor cells derived from adipose, muscle, and bone marrow from wild-type or craniosynostotic rabbits.
复制标题
DOI：
10.1097/prs.0b013e3181fad311
复制
发表时间：
2011-01
影响因子：
3.6
通讯作者：
Losee JE
中科院分区：
文献类型：
--
作者：
Cooper GM;Durham EL;Cray JJ Jr;Bykowski MR;DeCesare GE;Smalley MA;Mooney MP;Campbell PG;Losee JE
报告已经确定了能够在骨髓，肌肉和脂肪组织中成骨分化的细胞，但很少有这些不同细胞类型的直接比较。此外，很少有人研究组织特异性病理学和来自看似无关组织的细胞之间的潜在联系。在这里，我们比较了从野生型兔或非综合征性颅缝早闭（定义为一条或多条颅缝过早融合）兔中分离的细胞。细胞来源于10日龄野生型兔（WT; n=17）或年龄匹配的家族性非综合征性颅缝早闭症兔（CS; n=18）的骨髓、脂肪和肌肉。用骨形态发生蛋白4（BMP 4）刺激细胞，并评估碱性磷酸酶表达和细胞增殖。在WT兔中，肌肉来源的细胞比脂肪或骨髓来源的细胞具有更多的碱性磷酸酶活性。CS兔骨髓和肌肉来源的细胞成骨能力明显高于WT。脂肪来源的细胞表现出没有显着差异。而肌肉来源的细胞是最成骨的WT兔，骨髓来源的细胞是最成骨的CS兔。结果表明，来自不同组织的细胞具有不同的分化潜能。此外，来自患有颅缝早闭的兔的细胞不同于野生型来源的细胞。有趣的是，来自颅缝早闭兔的细胞与野生型细胞相比并不一致，这表明特定组织来源的细胞在颅缝早闭个体中的反应可能不同。
Reports have identified cells capable of osteogenic differentiation in bone marrow, muscle, and adipose tissues, but there are few direct comparisons of these different cell-types. Also, few have investigated the potential connection between a tissue-specific pathology and cells derived from seemingly unrelated tissues. Here, we compare cells isolated from wild-type rabbits or rabbits with nonsyndromic craniosynostosis, defined as the premature fusion of one or more of the cranial sutures. Cells were derived from bone marrow, adipose, and muscle of 10 day-old wild-type rabbits (WT; n=17) or from age-matched rabbits with familial nonsyndromic craniosynostosis (CS; n=18). Cells were stimulated with bone morphogenetic protein 4 (BMP4) and alkaline phosphatase expression and cell proliferation were assessed. In WT rabbits, cells derived from muscle had more alkaline phosphatase activity than cells derived from either adipose or bone marrow. The cells derived from CS rabbit bone marrow and muscle were significantly more osteogenic than WT. Adipose-derived cells demonstrated no significant differences. While muscle-derived cells were most osteogenic in WT rabbits, bone marrow-derived cells were most osteogenic in CS rabbits. Results suggest that cells from different tissues have different potentials for differentiation. Furthermore, cells derived from rabbits with craniosynostosis were different from wild-type derived cells. Interestingly, cells derived from the craniosynostotic rabbits were not uniformly more responsive compared with wild-type cells, suggesting that specific tissue-derived cells may react differently in individuals with craniosynostosis.