An anti body-conjugated internalizing quantum dot suitable for long-term live imaging of cells
An anti body-conjugated internalizing quantum dot suitable for long-term live imaging of cells
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DOI：
10.1139/o06-205
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发表时间：
2007-02-01
期刊：
影响因子：
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通讯作者：
Wadhwa, Renu
中科院分区：
文献类型：
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作者：
Kaul, Zeenia;Yaguchi, Tomoko;Wadhwa, Renu
量子点（QD）是荧光半导体纳米晶体，其作为生物应用中使用的常规有机染料的上级替代品而出现。尽管QD提供了优于常规荧光染料的几个优点，包括更大的光稳定性和更宽范围的激发和（或）发射波长，但它们的毒性在其作为分析、诊断和治疗工具的更广泛使用中一直是一个问题。我们制备了具有内化抗体的缀合物QD，并证明QD-抗体缀合物被有效内化到细胞中，并且即使在多次分裂后也是可见的。我们证明了内化的QD对细胞无毒，并为长期分子成像提供了一种敏感的工具。
Quantum dots (QD) are fluorescent semiconductor nanocrystals that are emerging as superior alternatives to the conventional organic dyes used in biological applications. Although QDs offer several advantages over conventional fluorescent dyes, including greater photostability and a wider range of excitation and (or) emission wavelengths, their toxicity has been an issue in its wider use as an analytic, diagnostic and therapeutic tool. We prepared a conjugate QD with an internalizing antibody and demonstrated that the QD-antibody conjugate is efficiently internalized into cells and is visible even after multiple divisions. We demonstrate that the internalized QD is nontoxic to cells and provides a sensitive tool for long-term molecular imaging.