Contrasting effects of an ultraviolet B and an ultraviolet A tanning lamp on interleukin-6, tumour necrosis factor-α and intercellular adhesion molecule-1 expression
Contrasting effects of an ultraviolet B and an ultraviolet A tanning lamp on interleukin-6, tumour necrosis factor-α and intercellular adhesion molecule-1 expression
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DOI：
10.1046/j.1365-2133.2001.04281.x
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发表时间：
2001-07-01
影响因子：
10.3
通讯作者：
Green, MHL
中科院分区：
文献类型：
--
作者：
Clingen, PH;Berneburg, M;Green, MHL
最近的研究表明，主要发射紫外线（UV） a的鞣制灯可诱导与皮肤癌发病相关的潜在诱变性DNA损伤（即环丁烷嘧啶二聚体）的大量产生。紫外线诱导的免疫抑制也是导致皮肤癌的重要事件。目的研究广谱UVB灯和以uva为主的鞣制灯对体外系统关键免疫分子的调节作用。方法比较了在广谱UVB灯和Philips“Performance”日晒灯照射下正常人表皮角质形成细胞中白细胞介素(IL)-6和肿瘤坏死因子(TNF)- α的分泌和mRNA表达，以及干扰素(IFN)- γ诱导的细胞内粘附分子(ICAM)-1的分泌和mRNA表达。结果广谱UVB照射后，照射后6 h细胞IL-6和tnf - α mRNA表达上调，照射后24 h细胞上清液中细胞因子呈剂量依赖性升高。相比之下，暴露于晒黑灯后，没有细胞因子分泌，也没有mRNA上调的证据。当细胞首先暴露于广谱UVB，然后暴露于晒黑灯时，UVB诱导的细胞因子分泌被抑制，尽管mRNA水平上调至接近单独暴露UVB时的水平。通过使用Schott WG 320 nm滤光片来减弱晒黑灯发出的UVB相对于UVA的水平，细胞因子分泌的抑制被证明与UVA暴露有关。两种紫外线源均以剂量依赖的方式抑制ifn - γ诱导的ICAM-1 mRNA表达。通过使用Schott WG 335 nm滤镜，发现鞣制灯对ICAM-1 mRNA表达的抑制与UVB暴露有关。结论不同波长的UVA和UVB对不同免疫调节分子的调节有不同的影响，表明不同波长的UVA和UVB之间可能存在相互作用。
Background Recent studies have demonstrated that a tanning lamp emitting predominantly ultraviolet (UV) A induces significant yields of the type of potentially mutagenic DNA damage that are associated with the onset of skin cancer (i.e. cyclobutane pyrimidine dimers). UV-induced immunosuppression is also an important event leading to skin cancer.Objectives To the modulation of key immunological molecules following exposure to a broad-spectrum UVB lamp and a predominantly UVA-emitting tanning lamp using model in vitro systems.Methods We compared secretion and mRNA expression of interleukin (IL)-6 and tumour necrosis factor (TNF)-alpha in normal human epidermal keratinocytes, and interferon (IFN)-gamma -induced intracellular adhesion molecule (ICAM)-1 in normal human fibroblasts irradiated in vitro with a broad-spectrum UVB lamp or with a Philips 'Performance' tanning lamp.Results With broad-spectrum UVB irradiation, upregulation of IL-6 and TNF-alpha mRNA was detected 6 h after irradiation, and a dose-dependent increase of cytokines in the supernatants of irradiated cells was found 24 h after irradiation. In contrast, there was no cytokine secretion and little evidence for mRNA upregulation following exposure to a tanning lamp. When cells were exposed first to broad-spectrum UVB, then the tanning lamp, UVB-induced cytokine secretion was inhibited, although mRNA levels were upregulated to a level close to that observed with UVB alone. By using a Schott WG 320 nm filter to attenuate the level of UVB relative to UVA emitted by the tanning lamp, the inhibition of cytokine secretion was shown to be associated with UVA exposure. Both UV sources inhibited IFN-gamma -induced ICAM-1 mRNA expression in a dose-dependent fashion. By using a Schott WG 335 nm filter, inhibition of ICAM-1 mRNA expression by the tanning lamp was shown to be associated with UVB exposure.Conclusions These results suggest that UV sources emitting different levels of UVA and UVB have differential effects on the modulation of different immunoregulatory molecules, and indicate that there are potential interactions between these wavelengths.