Comparative Analysis of Tissue Tropism of Bipartite Geminiviruses
Comparative Analysis of Tissue Tropism of Bipartite Geminiviruses
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二分体双生病毒组织嗜性的比较分析
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
H. Jeske
中科院分区：
文献类型：
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作者：
C. Wege;K. Saunders;J. Stanley;H. Jeske
苘麻花叶病毒（AbMV）是苘麻病毒属的二分双生病毒，已在严格韧皮部限制的苘麻中无性繁殖多年。利用原位杂交和免疫学分析，将实验室宿主本塞姆氏烟草中 AbMV 的组织向性与其他两种二分贝戈莫病毒、非洲木薯花叶病毒 (ACMV) 和番茄金花叶病毒 (TGMV) 进行比较。对第一批全身感染的叶子以及腋生和花芽的纵向切片的分析表明，所有三种病毒最初都局限于维管束痕迹，尽管后来在几乎所有组织类型中都可以检测到 ACMV 和 TGMV。相比之下，在整个感染过程中，AbMV 在该宿主体内始终严格限制于韧皮部。与 AbMV 感染相关的轻微症状相比，ACMV 和 TGMV 移出本塞姆氏烟草韧皮部组织的能力与严重症状的发展相关。研究还表明，与 AbMV 密切相关的 Sida micrantha 花叶病毒在小花锦葵中的韧皮部有限，尽管它会在该宿主中引起严重的卷叶、发育迟缓和坏死。目前的数据表明，二分体病毒可以表现出截然不同的组织趋向性模式。
Abutilon mosaic virus (AbMV), a bipartite geminivirus of the genus Begomovirus, has been vegetatively propagated for many years in Abutilon sellovianum in which it is strictly phloem-restricted. Using in situ hybridization and immunological analyses, the tissue tropism of AbMV in the laboratory host Nicotiana benthamiana was compared with that of two other bipartite begomoviruses, African cassava mosaic virus (ACMV) and tomato golden mosaic virus (TGMV). Analysis of the first systemically infected leaves and longitudinal sections of axillary and flower buds revealed that all three viruses are initially confined to the vascular traces, although both ACMV and TGMV are later detectable in nearly all tissue types. In contrast, AbMV remained strictly phloem-limited in this host throughout the course of infection. The ability of ACMV and TGMV to move out of N. benthamiana phloem tissues is correlated with the development of severe symptoms in comparison with the mild symptoms associated with AbMV infection. It was also demonstrated that Sida micrantha mosaic virus, a virus that is closely related to AbMV, is phloem-limited in Malva parviflora even though it induces severe leaf curl, stunting and necrosis in this host. The present data demonstrate that bipartite begomoviruses can exhibit strikingly different patterns of tissue tropism.