Melatonin regulating the expression of miRNAs involved in hair follicle cycle of cashmere goats skin
Melatonin regulating the expression of miRNAs involved in hair follicle cycle of cashmere goats skin
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DOI：
10.3724/sp.j.1005.2014.1235
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发表时间：
2014-12-01
期刊：
影响因子：
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通讯作者：
Zhang, Wenguang
中科院分区：
文献类型：
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作者：
Fu, Shaoyin;Zhao, Hongli;Zhang, Wenguang
褪黑激素和mieroRNAs（miRNAs）在调节毛囊发育中起重要作用。然而，褪黑激素对绒山羊皮肤和毛囊中miRNAs表达模式的影响在很大程度上还不清楚。为探讨褪黑素通过miRNA介导影响绒山羊生长的机制，对褪黑素埋植在内蒙古绒山羊体内的效果进行了研究。在实验中，5只一岁的母兔被植入褪黑激素，其余5只作为对照组。通过逆转录-实时聚合酶链反应（RT-qPCR）定量6种候选miRNA的表达。结果表明，褪黑激素显著改变了miRNAs的表达模式。除let-7a外，miR-203、miR-205、miR-96、miR-183和miR-199 a的表达水平在一个羊绒周期中发生三次转变;褪黑素改变了miRNA的共表达模式。对照组miRNA之间的相关系数为0.87-0.99（P
Melatonin and mieroRNAs (miRNAs) play important roles in regulating hair follicle development. However, the effect of melatonin on the expression pattern of miRNAs in skin and follicle of cashmere goats remain largely undefined. To explore the mechanism of melatonin affecting cashmere growth mediated by miRNAs, the effect of melatonin implants administered in Nei Mongol cashmere goats was assessed. In the experiment, five yearling does were implanted with melatonin, with the remaining other five females as control group. The expression of six candidate miRNAs was quantified by reverse transcription-real time polymerase chain reaction (RT-qPCR). The results indicated that melatonin significantly altered the expression pattern of miRNAs. Except for let-7a, the expression levels of miR-203, miR-205, miR-96, miR-183 and miR-199a occur three transitions during a cashmere cycle; melatonin changed the co-expression pattern of miRNAs. The correlation coefficient between miRNAs is 0.87-0.99 in control group(P