Cross-linked dry bonding: A new etch-and-rinse technique
Cross-linked dry bonding: A new etch-and-rinse technique
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DOI：
10.1016/j.dental.2016.06.014
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发表时间：
2016-09-01
期刊：
影响因子：
5
通讯作者：
Pashley, David H.
中科院分区：
文献类型：
--
作者：
Zhou, Jianfeng;Chiba, Ayaka;Pashley, David H.
目标。目的：确定酸蚀处理后的交联牙本质能否在不降低体外粘结强度的情况下脱水。使用拔除的人类第三磨牙，对照酸蚀牙本质与Single Bond Plus粘接，使用湿粘结或干粘结技术。实验酸蚀牙本质用5%葡萄籽提取物(GSE)在不同的溶剂中处理1min，然后用Single Bond Plus进行湿法和干法树脂-牙本质粘接。完全脱矿的牙本质梁用5%的GSE处理0、1或10min，然后用三点弯曲法测量硬度。其他完全脱矿的BEAM同样处理，然后在缓冲液中孵育1周，以测定内源性牙本质蛋白对胶原的增溶作用。24小时微拉伸粘结强度(MUTBS)湿组和干组分别为53.5+/-3.6和9.4+/-1.8 Mpa(p<；0.05)。水中5%GSE的MUTBS分别为53.7+/-3.4和39.1+/-9.7 Mpa(P
Objective. To determine if acid-etched, cross-linked dentin can be dehydrated without lowering bond strength below that of cross-linked wet-bonded dentin in vitro.Methods. Using extracted human third molars, control acid-etched dentin was bonded with Single Bond Plus, using either the wet- or dry-bonding technique. Experimental acid-etched dentin was treated with 5 mass% grape seed extract (GSE) in different solvents for 1 min before undergoing wet vs dry resin-dentin bonding with Single Bond Plus. Completely demineralized dentin beams were treated with 5% GSE for 0, 1 or 10 min, before measuring stiffness by 3-point flexure. Other completely demineralized beams were treated similarly and then incubated in buffer for 1 week to measure the collagen solubilization by endogenous dentin proteases.Results. 24h microtensile bond strengths (mu TBS) in wet and dry controls were 53.5 +/- 3.6 and 9.4 +/- 1.8 MPa, respectively (p < 0.05). 5% GSE in water gave mu TBS of 53.7 +/- 3.4 and 39.1 +/- 9.7 MPa (p