The relationship between genetic and cytogenetic maps of pea .1. Standard and translocation karyotypes
The relationship between genetic and cytogenetic maps of pea .1. Standard and translocation karyotypes
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DOI：
10.1139/g97-797
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发表时间：
1997-10-01
期刊：
影响因子：
3.1
通讯作者：
Ellis, THN
中科院分区：
文献类型：
--
作者：
Hall, KJ;Parker, JS;Ellis, THN
对豌豆自交系及其杂种F1进行了详细的细胞遗传学研究，以探讨细胞遗传图谱与分子连锁图谱之间的关系。本文给出了豌豆标准系JI15、易位系JI61和作图群体用系JI281的有丝分裂核型。有丝分裂染色体的细胞遗传学分析检测到的染色体重排已进一步确定联会复合体（SC）分析和中期I染色体行为的研究。这种减数分裂分析允许SC物理长度的比较，观察到的交叉频率和重组频率，估计从遗传图谱，作为比较物理和遗传距离的一种手段。
A detailed cytogenetical study of inbred lines of pea and their F-1 hybrids has been undertaken to study the relationship between the cytogenetic map and the molecular linkage map. The mitotic karyotypes of a standard pea line, JI15, a translocation line, JI61, and line JI281, a line used in the production of a mapping population, are given. A chromosome rearrangement detected by cytogenetic analysis of mitotic chromosomes has been further defined by synaptonemal complex (SC) analysis and the study of metaphase I chromosome behaviour. This meiotic analysis has allowed a comparison of SC physical lengths, observed chiasma frequencies, and recombination frequencies, as estimated from the genetic map, as a means of comparing physical and genetic distances.