Colloidal solution of water in organic solvents: a microheterogeneous medium for enzymatic reactions.
Colloidal solution of water in organic solvents: a microheterogeneous medium for enzymatic reactions.
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水在有机溶剂中的胶体溶液：用于酶促反应的微异质介质。
作者：
K. Martínek;A. V. Levashov;Y. Khmelnitsky;N. Klyachko;I. Berezin
为了在体外模拟酶在体内的作用条件，酶分子被包裹在有机溶剂中表面活性剂的水合反胶束中。在该体系中，所研究的一种酶（过氧化物酶）的催化活性比在水中高得多，而另一种酶（醇脱氢酶）的特异性显著改变。
To simulate in vitro the conditions under which enzymes act in vivo, enzyme molecules have been entrapped in hydrated reverse micelles of a surfactant in organic solvents. In this system the catalytic activity of one of the enzymes studied (peroxidase) became much higher than in water, and the specificity of the other enzyme (alcohol dehydrogenase) was dramatically altered.