The Functional Difference of Dendritic Cells in HBeAg Negative Chronic Hepatitis B Patients with Three Different Spleen Deficiency Syndromes and the Therapeutic Evaluation of Chinese Medicine Intervention Based on Syndrome Differentiation.
The Functional Difference of Dendritic Cells in HBeAg Negative Chronic Hepatitis B Patients with Three Different Spleen Deficiency Syndromes and the Therapeutic Evaluation of Chinese Medicine Intervention Based on Syndrome Differentiation.
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HBeAg阴性慢性乙型肝炎三种脾虚证患者树突状细胞的功能差异及中医辨证干预的疗效评价
DOI：
10.1155/2014/802402
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Ji G
中科院分区：
文献类型：
--
作者：
Wang L;Zhang L;Feng X;Xing L;Zhang W;Jiang K;Song H;Ji G
Objective.探讨不同脾虚证型HBeAg阴性慢性B型肝炎（CH B）患者树突状细胞（DC）成熟度的差异，探讨中医辨证分型在疗效评价中的作用。方法.研究对象120例，分为脾虚肝郁证、脾虚湿热证、脾虚肾虚证3个治疗组和1个健康对照组，每组30例。给予相应药物治疗。结果指标包括DC表型、肝功能、IL-10、IL-12和HBV-DNA水平。结果各组成熟DC表面标志物及细胞因子水平不同，SDKD组CD 80、CD 1a、HLA-DR、CD 1a阳性率最低。干预3个月后，治疗组CD 80、CD 86、CD 1a、HLA-DR、IL-12的表达明显升高，ALT、AST、IL-10的表达明显降低（P < 0.05）。SDKD组和SDLD组HBV-DNA水平均显著降低（P < 0.05）。结论. HBeAg阴性患者存在DCs发育不良，且不同证型间存在差异。中医辨证干预可促进DC成熟和功能的发挥，提高疗效。
Objective. To investigate the dendritic cells (DCs) maturity differences of HBeAg negative chronic hepatitis B (CHB) patients with different spleen deficiency (SD) syndromes and explore the role of syndrome differentiation in the therapeutic evaluation of Chinese medicine. Methods. 120 participants were recruited including three treatment groups in different SD syndrome categories as spleen deficiency with liver depression (SDLD), spleen deficiency with damp heat (SDDH), and spleen deficiency with kidney deficiency (SDKD) and one healthy control group; each group had 30 participants. Corresponding drugs were applied. The outcome measures included DC phenotype, liver function, IL-10, IL-12, and HBV-DNA levels. Results. The surface markers of mature DCs and cytokines levels were different in each group; the positive rate of CD80, CD1a, HLA-DR, and CD1a was the lowest in SDKD group. After 3-month intervention, the expression of CD80, CD86, CD1a, HLA-DR, and IL-12 significantly increased, while ALT, AST, and IL-10 significantly decreased (P < 0.05) in treatment groups. HBV-DNA level also significantly reduced in both SDKD and SDLD groups (P < 0.05). Conclusions. HBeAg negative patients had DCs dysmaturity, and there were differences between different SD syndromes. Chinese medicine intervention according to syndrome differentiation could advance the maturity and function of DCs and improve the therapeutic effect.
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影响因子：
8.6
作者：
Chen, HW;Liao, CH;Lin, CM
通讯作者：
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影响因子：
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DOI：
10.1016/j.bbadis.2011.09.013
发表时间：
2011-12-01
影响因子：
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作者：
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通讯作者：
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影响因子：
2.5
作者：
Zheng, BJ;Zhou, J;Wen, YM
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