Substance P and calcitonin gene-related peptide increase IL-1β, IL-6 and TNFα secretion from human peripheral blood mononuclear cells
Substance P and calcitonin gene-related peptide increase IL-1β, IL-6 and TNFα secretion from human peripheral blood mononuclear cells
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DOI：
10.1016/s0197-0186(01)00094-8
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发表时间：
2002-04-01
影响因子：
4.2
通讯作者：
Suarez-Roca, H
中科院分区：
文献类型：
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作者：
Cuesta, MC;Quintero, L;Suarez-Roca, H
我们发现P物质(SP)和降钙素基因相关肽(CGRP)(0.3-1um)以浓度依赖的方式增加培养的人外周血单个核细胞分泌IL-1β(IL-1β)、IL-6(IL-6)和肿瘤坏死因子α(TNFα)。P物质和降钙素基因相关肽(0.1um)协同促进肿瘤坏死因子α的基础分泌。强啡肽A((1-17))(0.1-1um)不影响基础细胞因子的分泌。脂多糖(10 ng/ml)诱导的细胞因子分泌和[H-3]胸腺嘧啶核苷摄取不受任何神经肽(0.1微米)的影响，因此，SP和CGRP仅在高浓度时刺激淋巴细胞产生促炎细胞因子，与组织损伤时相似。(C)2002年由爱思唯尔科学有限公司出版。
We found that substance P (SP) and calcitonin gene-related peptide (CGRP) (0.3-1 muM) increased, in a concentration-dependent manner, the basal secretion of interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and tumor necrosis factor alpha (TNFalpha) from cultured lymphocyte-enriched mononuclear cells isolated from human peripheral blood. SP and CGRP (0.1 muM) synergistically increased basal TNFalpha secretion. Dynorphin A((1-17)) (0.1-1 muM) did not modify basal cytokine secretion. Lipopolysacharide (10 ng/ml)-induced cytokine secretion and [H-3]thymidine uptake were not altered by any neuropeptide (at 0.1 muM) Thus, SP and CGRP stimulate the production of pro-inflammatory cytokines from lymphocytes only at high concentrations, similar to those reached during tissue damage. (C) 2002 Published by Elsevier Science Ltd.