Trichoderma harzianum-induced resistance against Fusarium oxysporum involves regulation of nuclear DNA content, cell viability and cell cycle-related genes expression in cucumber roots
Trichoderma harzianum-induced resistance against Fusarium oxysporum involves regulation of nuclear DNA content, cell viability and cell cycle-related genes expression in cucumber roots
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DOI：
10.7512/j.issn.1001-2303.2017.05.09
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发表时间：
2017-01-01
影响因子：
1.8
通讯作者：
Ahammed, Golam-Jalal
中科院分区：
文献类型：
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作者：
Chen, Shuang-Chen;Zhao, Hong-Jiao;Ahammed, Golam-Jalal
黄瓜枯萎病是黄瓜的毁灭性病害之一，利用哈茨木霉等生防菌可以有效地防治黄瓜枯萎病。然而，控制T.在黄瓜植物中，哈茂诱导的增强的抗性在很大程度上仍然未知。在这里，我们筛选了有效的T。哈茨木分离物TH 58能有效防治黑胫病。尖孢菌（FO）。温室功效试验还显示，TH 58降低了69.7%的发病率。FO诱导了根细胞活性氧的过度积累，而TH 58则抑制了根细胞活性氧的过度积累，提高了根细胞的活力。尖孢菌感染TH 58接种可逆转FO诱导的细胞分裂阻滞，并通过诱导2C组分调节细胞核DNA含量的比例分布。此外，与单独感染FO相比，TH 58预接种的幼苗中细胞周期相关基因CDKA、CDKB、CycA、CycB、CycD 3;1和CycD 3;2的表达水平上调。综上所述，这些结果表明，T。哈茨木通过改变细胞核DNA含量和细胞周期相关基因表达来提高黄瓜幼苗对枯萎病的抗性，从而维持较低的活性氧积累和较高的根细胞活力。
Fusarium wilt, one of the destructive diseases of cucumber can be effectively controlled by using biocontrol agents such as Trichoderma harzianum. However, the mechanisms controlling T. harzianum-induced enhanced resistance remain largely unknown in cucumber plants. Here we screened the potent T. harzianum isolate TH58 that could effectively control F. oxysporum (FO). Glasshouse efficacy trials also showed that TH58 decreased disease incidence by 69.7 %. FO induced ROS over accumulation, while TH58 inoculation suppressed ROS over accumulation and improved root cell viability under F. oxysporum infection. TH58 inoculation could reverse the FO-induced cell division block and regulate the proportional distribution of nuclear DNA content through inducing 2C fraction. Moreover, the expression levels of cell cycle-related genes such as CDKA, CDKB, CycA, CycB, CycD3;1 and CycD3;2 in TH58 - pre-inoculated seedlings were up-regulated compared with those infected with FO alone. Taken together, these results suggest that T. harzianum improved plant resistance against Fusarium wilt disease via alterations in nuclear DNA content and cell cycle-related genes expression that might maintain a lower ROS accumulation and higher root cell viability in cucumber seedlings.