Charge-conversional polyethylenimine-entrapped gold nanoparticles with 131I-labeling for enhanced dual mode SPECT/CT imaging and radiotherapy of tumors
Charge-conversional polyethylenimine-entrapped gold nanoparticles with 131I-labeling for enhanced dual mode SPECT/CT imaging and radiotherapy of tumors
复制标题
带有 (131)I 标记的电荷转换聚乙烯亚胺包埋金纳米粒子，用于增强双模式 SPECT/CT 成像和肿瘤放射治疗。
DOI：
10.1039/d0bm00649a
复制
发表时间：
2020
影响因子：
6.6
通讯作者：
Jinhua Zhao
中科院分区：
文献类型：
--
作者：
Jingyi Zhu;Lingzhou Zhao;Pingping Zhao;Junxing Yang;Jianhui Shi;Jinhua Zhao
新型治疗诊断纳米系统显示出同时实现及时诊断和有效治疗的巨大潜力。然而，由于治疗诊断纳米系统在肿瘤部位的积累相对较低，治疗诊断效率受到限制。在这项研究中，构建了一种具有 pH 响应电荷转换特性的新型治疗诊断纳米系统，以改善癌细胞对癌细胞的摄取，以增强单光子发射计算机断层扫描 (SPECT)/计算机断层扫描 (CT) 双模式成像和肿瘤放射治疗。具体来说，聚乙烯亚胺（PEI）被用作纳米平台，与具有N-羟基琥珀酰亚胺末端的聚乙二醇（PEG）单甲醚（mPEG-NHS）、具有马来酰亚胺和琥珀酰亚胺基戊酸酯末端的PEG（MAL-PEG-SVA）、烷氧基苯基酰基磺酰胺（APAS）、3-（4'-羟苯基）丙酸-OSu（HPAO）和依次使用异硫氰酸荧光素 (FI)。然后利用形成的功能化 PEI 来捕获金纳米颗粒，乙酰化 PEI 的剩余胺，并用放射性碘 131 (131I) 进行标记，以构建治疗诊断纳米系统。结果表明，治疗诊断纳米系统具有 3.8 nm Au 核心，并表现出优异的胶体稳定性。由于 APAS 的电荷转换特性，APAS 连接的 PEI 包埋的金纳米颗粒可以在微酸性微环境中从中性转变为带正电，从而改善细胞摄取。最重要的是，经过 131I 标记后，所生成的治疗诊断纳米系统可以实现增强的 SPECT/CT 双模式成像和体外癌细胞的放射治疗以及体内异种移植肿瘤模型。构建的APAS连接的PEI纳米系统具有作为各种癌症的SPECT/CT成像和放射治疗模型的巨大潜力。
Novel theranostic nanosystems demonstrate great potential to achieve timely diagnosis and effective therapy at the same time. However, due to the relatively low accumulation of theranostic nanosystems at the tumor site, the theranostic efficiency is limited. In this study, a novel theranostic nanosystem with a pH-responsive charge conversion property was constructed to improve the cellular uptake towards cancer cells for enhanced single photon emission computed tomography (SPECT)/computed tomography (CT) dual mode imaging and radiotherapy of tumors. In detail, polyethylenimine (PEI) was utilized as a nanoplatform to link with polyethylene glycol (PEG) monomethyl ether with one end of N-hydroxylsuccinimide (mPEG-NHS), PEG with ends of maleimide and succinimidyl valerate (MAL-PEG-SVA), alkoxyphenyl acylsulfonamide (APAS), 3-(4′-hydroxyphenyl)propionic acid-OSu (HPAO), and fluorescein isothiocyanate (FI), successively. The formed functionalized PEI was then utilized to entrap gold nanoparticles, acetylate the remaining amines of PEI and label with radioactive iodine-131 (131I) to build theranostic nanosystems. The result demonstrated that the theranostic nanosystem has a 3.8 nm Au core and showed excellent colloidal stability. On account of the charge conversion property of APAS, the APAS linked PEI entrapped gold nanoparticles could switch from neutral to positive in a slightly acidic microenvironment, which induced improved cellular uptake. Above all, after 131I labeling, the generated theranostic nanosystem could achieve enhanced SPECT/CT dual mode imaging and radiotherapy of cancer cells in vitro and a xenograft tumor model in vivo. The constructed APAS-linked PEI nanosystem has great potential to be used as a model for SPECT/CT imaging and radiotherapy of various types of cancer.