Recombinant Soluble Henipavirus Glycoprotein Preparation.
Recombinant Soluble Henipavirus Glycoprotein Preparation.
复制标题
重组可溶性亨尼帕病毒糖蛋白制剂。
DOI：
10.1007/978-1-0716-3283-3_3
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Broder,ChristopherC
中科院分区：
文献类型：
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作者：
Yan,Lianying;Sterling,SpencerL;Fusco,DeborahL;Chan,Yee-Peng;Xu,Kai;Laing,EricD;Broder,ChristopherC
亨尼帕病毒具有两种包膜糖蛋白，即介导细胞进入的附着（G）和融合（F）蛋白，并且是病毒中和抗体应答的主要靶标。重组表达技术已用于产生保留天然样寡聚体构象和表位的可溶性G和F蛋白（sG和sF），这有利于疫苗和抗病毒抗体治疗剂的开发和表征。除了亨德拉病毒和尼帕病毒四聚体sG和三聚体sF的生产，我们还描述了雪松病毒四聚体sG和加纳病毒三聚体sF糖蛋白的表达和纯化。这些亨尼帕病毒的糖蛋白也被用作免疫抗原，以产生单克隆抗体，并证明与泛亨尼帕病毒多重微球免疫测定结合。
Henipaviruses possess two envelope glycoproteins, the attachment (G) and the fusion (F) proteins that mediate cellular entry and are the major targets of virus-neutralizing antibody responses. Recombinant expression technologies have been used to produce soluble G and F proteins (sG and sF) that retain native-like oligomeric conformations and epitopes, which are advantageous for the development and characterization of vaccines and antiviral antibody therapeutics. In addition to Hendra virus and Nipah virus tetrameric sG and trimeric sF production, we also describe the expression and purification of Cedar virus tetrameric sG and Ghana virus trimeric sF glycoproteins. These henipavirus glycoproteins were also used as immunizing antigens to generate monoclonal antibodies, and binding was demonstrated with a pan-henipavirus multiplex microsphere immunoassay.