RNA sequencing of pancreatic circulating tumour cells implicates WNT signalling in metastasis.
RNA sequencing of pancreatic circulating tumour cells implicates WNT signalling in metastasis.
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DOI：
10.1038/nature11217
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发表时间：
2012-07-26
期刊：
影响因子：
64.8
通讯作者：
Haber, Daniel A.
中科院分区：
文献类型：
--
作者：
Yu, Min;Ting, David T.;Stott, Shannon L.;Wittner, Ben S.;Ozsolak, Fatih;Paul, Suchismita;Ciciliano, Jordan C.;Smas, Malgorzata E.;Winokur, Daniel;Gilman, Anna J.;Ulman, Matthew J.;Xega, Kristina;Contino, Gianmarco;Alagesan, Brinda;Brannigan, Brian W.;Milos, Patrice M.;Ryan, David P.;Sequist, Lecia V.;Bardeesy, Nabeel;Ramaswamy, Sridhar;Toner, Mehmet;Maheswaran, Shyamala;Haber, Daniel A.
Circulating tumour cells (CTCs) shed into blood from primary cancers include putative precursors that initiate distal metastases. While these cells are extraordinarily rare, they may identify cellular pathways contributing to the blood-borne dissemination of cancer. Here, we adapted a microfluidic device for efficient capture of CTCs from an endogenous mouse pancreatic cancer model and subjected CTCs to single molecule RNA sequencing, identifying Wnt2 as a candidate gene enriched in CTCs. Expression of Wnt2 in pancreatic cancer cells suppresses anoikis, enhances anchorage-independent sphere formation, and increases metastatic propensity in vivo. This effect is correlated with fibronectin upregulation and suppressed by inhibition of Map3k7 (Tak1) kinase. In humans, formation of non-adherent tumour spheres by pancreatic cancer cells is associated with upregulation of multiple Wnt genes, and pancreatic CTCs revealed enrichment for Wnt signaling in 5 of 11 cases. Thus, molecular analysis of CTCs may identify candidate therapeutic targets to prevent the distal spread of cancer.
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影响因子：
10.5
作者：
Dontu, G;Abdallah, WM;Wicha, MS
通讯作者：
Wicha, MS
影响因子：
11.2
作者：
Hoshida Y;Nijman SM;Kobayashi M;Chan JA;Brunet JP;Chiang DY;Villanueva A;Newell P;Ikeda K;Hashimoto M;Watanabe G;Gabriel S;Friedman SL;Kumada H;Llovet JM;Golub TR
通讯作者：
Golub TR
DOI：
10.1126/science.1200801
发表时间：
2011-02-04
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ting DT;Lipson D;Paul S;Brannigan BW;Akhavanfard S;Coffman EJ;Contino G;Deshpande V;Iafrate AJ;Letovsky S;Rivera MN;Bardeesy N;Maheswaran S;Haber DA
通讯作者：
Haber DA
影响因子：
2.7
作者：
Reynolds, BA;Weiss, S
通讯作者：
Weiss, S
影响因子：
4.8
作者：
Ninomiya-Tsuji, J;Kajino, T;Matsumoto, K
通讯作者：
Matsumoto, K