Silence of yki by miR-7 regulates the Hippo pathway
Silence of yki by miR-7 regulates the Hippo pathway
复制标题
miR-7 沉默 yki 调节 Hippo 通路
DOI：
10.1016/j.bbrc.2020.08.069
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发表时间：
2020
影响因子：
3.1
通讯作者：
Zhou Zizhang
中科院分区：
文献类型：
--
作者：
Yu Xuan;Li Mingming;Cui Meng;Sun Bing;Zhou Zizhang
The Hippo signaling pathway governs organ size via coordinating cell proliferation and apoptosis, and its dysregulation causes congenital diseases and cancers. The homeostasis of Hippo pathway is achieved through multiple post translational modifications. ThroughDrosophilagenetic screening, we found that miRNAs were also involved in Hippo pathway regulation. Here, we showed that overexpression of miR-7 resulted in small wings, which were neutralized by miR-7-sponge (miR-7-sp) co-expression. Mechanistically, miR-7 inhibited the expression of Hippo pathway target genes. Epistatic analyses revealed that miR-7 modulated Hippo pathway through the transcriptional cofactor Yorkie (Yki). Consistently, overexpression of miR-7 decreased Yki protein. We further found a seed sequence of miR-7 in theyki3′-UTR region. In addition, we discovered that miR-7 was a transcriptional target of Yki. Thus, a negative feedback loop existed for fine tuning Hippo pathway activity. Taken together, our findings uncovered a novel mechanism by which Yki was silenced by miR-7 for Hippo pathway regulation.
