High deleterious genomic mutation rate in stationary phase of Escherichia coli
High deleterious genomic mutation rate in stationary phase of Escherichia coli
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DOI：
10.1126/science.1087911
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发表时间：
2003-11-28
期刊：
影响因子：
56.9
通讯作者：
Scherer, S
中科院分区：
文献类型：
--
作者：
Loewe, L;Textor, V;Scherer, S
在自然栖息地中，细菌大部分时间都处于某种形式的生长停滞状态。人们对这些阶段的有害突变知之甚少，因此对发生的进化事件的理解有限。在大肠杆菌长时间稳定期的有害突变积累实验中，每天每个基因组观察到约0.03个轻微有害突变。这比快速生长细胞的外推高了一个数量级，但与自适应稳定期突变实验中的观察结果一致。这些发现可能会影响对细菌进化和细菌致病性出现的理解。
In natural habitats, bacteria spend most of their time in some form of growth arrest. Little is known about deleterious mutations in such stages, and consequently there is limited understanding of what evolutionary events occur. In a deleterious mutation accumulation experiment in prolonged stationary phase of Escherichia coli, about 0.03 slightly deleterious mutations were observed per genome per day. This is over an order of magnitude higher than extrapolations from fast-growing cells, but in line with inferences from observations in adaptive stationary phase mutation experiments. These findings may affect understanding of bacterial evolution and the emergence of bacterial pathogenicity.