Human Serum Supplementation Promotes Streptococcus mitis Growth and Induces Specific Transcriptomic Responses.
Human Serum Supplementation Promotes Streptococcus mitis Growth and Induces Specific Transcriptomic Responses.
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人血清补充剂可促进轻症链球菌生长并诱导特异性转录组反应。
DOI：
10.1128/spectrum.05129-22
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发表时间：
2023-06-15
影响因子：
3.7
通讯作者：
Palmer, Kelli L.
中科院分区：
文献类型：
--
作者：
Wei, Yahan;Sturges, Camille I.;Palmer, Kelli L.
缓症链球菌是人类口腔微生物群的正常成员，也是引起感染性心内膜炎（IE）的主要机会病原体。尽管S. mitis和人类宿主，了解S.缓和的生理和适应宿主相关环境的机制是不够的，特别是与其他IE细菌病原体相比。本研究报告了人血清对S.缓症和其他致病性链球菌，包括链球菌。oralis，S.肺炎链球菌和S.无乳。使用转录组学分析，我们发现，加入人血清，S。缓症下调金属离子和糖的摄取系统、脂肪酸生物合成基因、以及参与应激反应和与生长和复制相关的其它过程的基因。S. mitis响应于人血清上调氨基酸和短肽的摄取系统。锌的可用性和诱导的短肽结合蛋白所感知的环境信号不足以赋予生长促进作用。需要进行更多的调查，以建立促进增长的机制。总之，我们的研究有助于对S.在宿主相关条件下的缓解生理学。重要性S.在人口腔和血流发病过程中，轻症暴露于人血清组分。然而，血清组分对该细菌的生理作用仍不清楚。利用转录组学分析，S.揭示了缓症链球菌对人血清的生物学反应过程，提高了对缓症链球菌的基本认识。人类宿主条件下的螨生理学。
Streptococcus mitis is a normal member of the human oral microbiota and a leading opportunistic pathogen causing infective endocarditis (IE). Despite the complex interactions between S. mitis and the human host, understanding of S. mitis physiology and its mechanisms of adaptation to host-associated environments is inadequate, especially compared with other IE bacterial pathogens. This study reports the growth-promoting effects of human serum on S. mitis and other pathogenic streptococci, including S. oralis, S. pneumoniae, and S. agalactiae. Using transcriptomic analyses, we identified that, with the addition of human serum, S. mitis downregulates uptake systems for metal ions and sugars, fatty acid biosynthetic genes, and genes involved in stress response and other processes related with growth and replication. S. mitis upregulates uptake systems for amino acids and short peptides in response to human serum. Zinc availability and environmental signals sensed by the induced short peptide binding proteins were not sufficient to confer the growth-promoting effects. More investigation is required to establish the mechanism for growth promotion. Overall, our study contributes to the fundamental understanding of S. mitis physiology under host-associated conditions. IMPORTANCE S. mitis is exposed to human serum components during commensalism in the human mouth and bloodstream pathogenesis. However, the physiological effects of serum components on this bacterium remain unclear. Using transcriptomic analyses, S. mitis biological processes that respond to the presence of human serum were revealed, improving the fundamental understanding of S. mitis physiology in human host conditions.
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