RNA-seq-based evaluation of bicolor tepal pigmentation in Asiatic hybrid lilies (Lilium spp.).
RNA-seq-based evaluation of bicolor tepal pigmentation in Asiatic hybrid lilies (Lilium spp.).
复制标题
基于RNA-seq的亚洲杂交百合（百合属）双色花被片色素沉着的评估。
DOI：
10.1186/s12864-016-2995-5
复制
发表时间：
2016-08-11
期刊：
影响因子：
4.4
通讯作者：
Matsuura H
中科院分区：
文献类型：
--
作者：
Suzuki K;Suzuki T;Nakatsuka T;Dohra H;Yamagishi M;Matsuyama K;Matsuura H
被子植物花朵的颜色图案是由空间和时间上受限的色素沉积产生的。识别限制色素沉积的机制是一个广泛关注的话题。一些双子叶植物物种会形成双色花瓣，这通常是由查尔酮合酶（CHS）基因的转录后基因沉默（PTGS）引起的。亚洲杂交百合（Lilium spp.）品种 Lollypop 发育出双色花被片，尖端有颜色，基部白色。在这里，我们分析了 Lollypop 的有色和无色花被片部分的整体转录，以确定主要的转录组差异。 RNA-seq 数据的从头组装产生了 49,239 个重叠群（39,426 个 unigenes），其中包括各种新的转录本，例如参与类黄酮糖基化和隔离以及花青素生物合成调节的转录本。此外，1258 个 unigene 在花被部分之间表现出显着差异表达（错误发现率 <0.05）。有色素的花被部分积累了更多的花青素，注释为花青素生物合成基因的unigenes（例如CHS、二氢黄酮醇4-还原酶和花青素合酶）的表达量比无色素部分高7-30倍。这些结果表明生物合成基因的转录调控更有可能参与双色百合花被片的发育，而不是CHS基因的PTGS。此外，与LhMYB12同源的unigene（通常调节百合中全花被花青素色素沉着）的表达水平在色素部分高出2倍以上。因此，LhMYB12 应该参与双色花被片生物合成基因的转录调控。还鉴定了可能抑制或增强花青素生物合成基因（包括 WD40 基因）表达的其他因素，并讨论了它们在双色发育中的参与。我们的研究结果表明棒棒糖花被片的双色性状是由花青素生物合成基因的转录调控引起的，LhMYB12的转录谱为阐明该性状的机制提供了线索。本研究中构建的花被转录组将加速对百合属花青素颜色模式（包括双色模式）的遗传控制的研究。本文的在线版本 (doi:10.1186/s12864-016-2995-5) 包含补充材料，可供授权用户使用。
Color patterns in angiosperm flowers are produced by spatially and temporally restricted deposition of pigments. Identifying the mechanisms responsible for restricted pigment deposition is a topic of broad interest. Some dicots species develop bicolor petals, which are often caused by the post-transcriptional gene silencing (PTGS) of chalcone synthase (CHS) genes. An Asiatic hybrid lily (Lilium spp.) cultivar Lollypop develops bicolor tepals with pigmented tips and white bases. Here, we analyzed the global transcription of pigmented and non-pigmented tepal parts from Lollypop, to determine the main transcriptomic differences. De novo assembly of RNA-seq data yielded 49,239 contigs (39,426 unigenes), which included a variety of novel transcripts, such as those involved in flavonoid-glycosylation and sequestration and in regulation of anthocyanin biosynthesis. Additionally, 1258 of the unigenes exhibited significantly differential expression between the tepal parts (false discovery rates <0.05). The pigmented tepal parts accumulated more anthocyanins, and unigenes annotated as anthocyanin biosynthesis genes (e.g., CHS, dihydroflavonol 4-reductase, and anthocyanidin synthase) were expressed 7–30-fold higher than those in non-pigmented parts. These results indicate that the transcriptional regulation of biosynthesis genes is more likely involved in the development of bicolor lily tepals rather than the PTGS of CHS genes. In addition, the expression level of a unigene homologous to LhMYB12, which often regulates full-tepal anthocyanin pigmentation in lilies, was >2-fold higher in the pigmented parts. Thus, LhMYB12 should be involved in the transcriptional regulation of the biosynthesis genes in bicolor tepals. Other factors that potentially suppress or enhance the expression of anthocyanin biosynthesis genes, including a WD40 gene, were identified, and their involvement in bicolor development is discussed. Our results indicate that the bicolor trait of Lollypop tepals is caused by the transcriptional regulation of anthocyanin biosynthesis genes and that the transcription profile of LhMYB12 provides a clue for elucidating the mechanisms of the trait. The tepal transcriptome constructed in this study will accelerate investigations of the genetic controls of anthocyanin color patterns, including the bicolor patterns, of Lilium spp. The online version of this article (doi:10.1186/s12864-016-2995-5) contains supplementary material, which is available to authorized users.