Serology characteristics of SARS-CoV-2 infection after exposure and post-symptom onset
Serology characteristics of SARS-CoV-2 infection after exposure and post-symptom onset
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DOI：
10.1183/13993003.00763-2020
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发表时间：
2020-08-01
影响因子：
24.3
通讯作者：
Chen, Yu
中科院分区：
文献类型：
--
作者：
Lou, Bin;Li, Ting-Dong;Chen, Yu
背景：SARS冠状病毒2型感染的及时诊断是治疗和预防的前提。其血清学特征和补体诊断价值有待进一步研究。方法：收集浙江大学杭州第一附属医院收集的80例聚合酶链式反应确诊的新冠肺炎患者的系列血清。结果：SARS-CoV-2抗体阳转率分别为98.8%、93.8%和93.8%。第一个可检测到的血清学标志物是抗体，其次是IgM和IgG，中位血清转换时间分别为暴露后15、18和20天(D.P.E.)或分别于发病后9、10和12天(D.P.O.)。从6DP.O开始，抗体水平迅速上升。并伴随着病毒载量的下降。在疾病早期(0~7天)，抗体的敏感性(64.1%)高于抗体(33.3%)和抗体(33.3%)；
Background: Timely diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is a prerequisite for treatment and prevention. The serology characteristics and complement diagnosis value of the antibody test to RNA test need to be demonstrated.Method: Serial sera of 80 patients with PCR-confirmed coronavirus disease 2019 (COVID-19) were collected at the First Affiliated Hospital of Zhejiang University, Hangzhou, China. Total antibody (Ab), IgM and IgG antibodies against SARS-CoV-2 were detected, and the antibody dynamics during the infection were described.Results: The seroconversion rates for Ab, IgM and IgG were 98.8%, 93.8% and 93.8%, respectively. The first detectible serology marker was Ab, followed by IgM and IgG, with a median seroconversion time of 15, 18 and 20 days post exposure (d.p.e.) or 9, 10 and 12 days post onset (d.p.o.), respectively. The antibody levels increased rapidly beginning at 6 d.p.o. and were accompanied by a decline in viral load. For patients in the early stage of illness (0-7 d.p.o), Ab showed the highest sensitivity (64.1%) compared with IgM and IgG (33.3% for both; p