SPONTANEOUS DELETIONS AND FLANKING REGIONS OF THE CHROMOSOMALLY INHERITED HEMOLYSIN DETERMINANT OF AN ESCHERICHIA-COLI O6-STRAIN
SPONTANEOUS DELETIONS AND FLANKING REGIONS OF THE CHROMOSOMALLY INHERITED HEMOLYSIN DETERMINANT OF AN ESCHERICHIA-COLI O6-STRAIN
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DOI：
10.1128/jb.154.3.1145-1152.1983
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发表时间：
1983-01-01
影响因子：
3.2
通讯作者：
GOEBEL, W
中科院分区：
文献类型：
--
作者：
HACKER, J;KNAPP, S;GOEBEL, W
溶血性E.大肠杆菌菌株536（O 6）以相对高的速率（10-3-10-4）繁殖自发溶血素阴性突变体。I型突变体缺乏分泌型（外部）和周质型（内部）溶血素活性（Hlyex-/Hlyin-），并且不显示甘露糖抗性血凝（Mrh-）; II型突变体为Hlyex-/Hlyin+和Mrh+。该菌株溶血素产生（hly）和甘露糖抗性血凝（mrh）的遗传决定因子位于染色体上。杂交实验表明，I型突变体的hly决定簇的各个部分的特异性DNA探针已经失去了总的hly决定簇; I型突变体缺乏只有一部分的hlyB是必不可少的运输溶血素跨外膜。使用含有质粒pHly 152编码的hly决定簇末端序列（与hlyB相邻）的探针，确定相关序列也位于大肠杆菌染色体hly决定簇的hylB远端的侧翼。coli536。在染色体和质粒hly决定簇的hlyC-和hlyB-远端附近发现了几个其他相似或相同的序列。
The hemolytic E. coli strains 536 (O6) propagates spontaneous hemolysin-negative mutants at relatively high rates (10-3-10-4). Type I mutants lack secreted (external) and periplasmic (internal) hemolysin activity (Hlyex-/Hlyin-) and show no mannose-resistant hemagglutination (Mrh-); type II mutants are Hlyex-/Hlyin+ and Mrh+. The genetic determinants for hemolysin production (hly) and for mannose-resistant hemagglutination (mrh) of this strain are located on the chromosome. Hybridization experiments with DNA probes specific for various parts of the hly determinant reveal that type I mutants have lost the total hly determinant; type I mutants lack only part of hlyB that is essential for transport of hemolysin across the outer membrane. Using a probe that contains the end sequence of the plasmid pHly152-encoded hly determinant (adjacent to hlyB), it was determined that a related sequence also flanks the hylB-distal end of the chromosomal hly determinant of E. coli 536. Several other similar or identical sequences are found in the vicinity of the hlyC- and the hlyB-distal ends of the chromosomal and the plasmid hly determinants.