EXPRESSION, LOCALIZATION AND DEVELOPMENTAL REGULATION OF INSULIN-LIKE GROWTH FACTOR-I MESSENGER-RNA IN RAT SUBMANDIBULAR-GLAND
EXPRESSION, LOCALIZATION AND DEVELOPMENTAL REGULATION OF INSULIN-LIKE GROWTH FACTOR-I MESSENGER-RNA IN RAT SUBMANDIBULAR-GLAND
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DOI：
10.1016/0003-9969(93)90006-8
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发表时间：
1993-08-01
影响因子：
3
通讯作者：
ISEKI, S
中科院分区：
文献类型：
--
作者：
AMANO, O;ISEKI, S
采用北方印迹法和寡核苷酸探针原位杂交法检测了成熟和发育大鼠颌下腺中胰岛素样生长因子I（IGF-I）的mRNA。在成熟的成年大鼠中，IGF-I mRNA在下颌下腺中的表达水平高于肝脏，并且主要定位于颗粒曲小管（GCT）细胞中。北方印迹上的4.7 kb mRNA在肝脏中仅轻微表达，被证明是GCT细胞中IGF-I转录物的主要大小种类，并且其水平随着GCT在腺体中的出生后发育而逐渐增加。此外，IGF-I的1.8 kb mRNA在整个腺泡和导管系统中也以低得多的水平表达，与年龄无关。这些结果揭示了IGF-I作为啮齿动物下颌下腺中产生的许多生物活性多肽之一的地位。
The mRNA for insulin-like growth factor I (IGF-I) in the submandibular gland of mature and developing rats was examined by Northern blotting and in situ hybridization with an oligonucleotide probe. In the mature adult rat, IGF-I mRNA was expressed at a higher level in the submandibular gland than in the liver, and was localized primarily in the granular convoluted tubule (GCT) cells. A 4.7-kb mRNA on Northern blots, which was expressed only slightly in the liver, proved to be the predominant size species of IGF-I transcripts in the GCT cells, and its level increased progressively with the postnatal development of GCTs in the gland. In addition, a 1.8-kb mRNA for IGF-I was also expressed at a much lower level throughout the acinar and duct systems, irrespective of age. These results have shed a light on the status of IGF-I as one of the many biologically active polypeptides that are produced in the rodent submandibular gland.