Effects of leptin administration on long-term selected fat mice
Effects of leptin administration on long-term selected fat mice
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DOI：
10.1017/s0016672397002814
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发表时间：
1997-06-01
期刊：
影响因子：
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通讯作者：
Hill, WG
中科院分区：
文献类型：
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作者：
Bunger, L;Hill, WG
为了评估对瘦素激素敏感性的遗传变化在促进对肥胖长期选择的反应中的作用，将瘦素给予长期脂肪选择的小鼠（F）和对照系（C）。这些品系在约15周龄时的脂肪百分比（脂肪%）几乎相差三倍。治疗组（T）动物从91至105日龄接受每天两次5 mg/kg瘦素的腹膜内注射;未治疗组（U）动物接受等体积的磷酸盐缓冲盐水注射。在试验结束时，与未处理动物相比，两个品系中处理的动物具有显著（P < 0.05）更低的平均体重、食物摄入和脂肪（脂肪%：CT 3%，CU 7.4%，FT 14.9%，FU 21.1%）。然而，[（CT-CU）-（FT-FU）]系之间的反应差异均不显著（P > 0.05）。FT动物（3-29%）的肥满度范围非常广（根据干物质含量估计），远高于FU（15-31%）、CT（0.7- 6.4%）和CU（2- 15%）动物。虽然对瘦素的敏感性仍然存在于脂肪线中，但在所使用的剂量水平下，动物之间的反应似乎有所不同。
To assess the role of genetic changes in sensitivity to leptin hormone in contributing to responses to long-term selection for fatness, leptin was administered to a long-term fat selected (F) and a control line (C) of mice. These lines differ almost three fold in their percentage of fat (fat%) at about 15 weeks of age. Treated (T) animals received twice-daily intraperitoneal injections of 5 mg/kg leptin from 91 to 105 days of age; untreated (U) animals received equivolume injections of phosphate-buffered saline. Treated compared with untreated animals in both lines had significantly (P < 0.05) lower mean body weight, food intake and fatness at the end of test (fat%: CT 3 %, CU 7.4 %, FT 14.9 %, FU 21.1 %). The differences in response between the lines [(CT-CU) - (FT-FU)] were all non-significant (P > 0.05), however. There was a very wide range of fatness (estimated from dry matter content) among FT animals (3-29%), much higher than in FU (15-31%), CT (0.7-6.4 %) and CU (2-15 %) animals. While sensitivity to leptin remains in the fat line, response appears to vary among animals at the dose level used.