The Effects of Pretreatment with Lidocaine or Bupivacaine on the Spatial and Temporal Expression of c-Fos Protein in the Spinal Cord Caused by Plantar Incision in the Rat
The Effects of Pretreatment with Lidocaine or Bupivacaine on the Spatial and Temporal Expression of c-Fos Protein in the Spinal Cord Caused by Plantar Incision in the Rat
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DOI：
10.1213/01.ane.0000104580.89717.a2
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发表时间：
2004-04
影响因子：
5.7
通讯作者：
Xiaohui Sun;M. Yokoyama;S. Mizobuchi;R. Kaku;H. Nakatsuka;Toru Takahashi;K. Morita
中科院分区：
文献类型：
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作者：
Xiaohui Sun;M. Yokoyama;S. Mizobuchi;R. Kaku;H. Nakatsuka;Toru Takahashi;K. Morita
研究了大鼠足底切开后脊髓背角c-Fos蛋白（Fos）表达的时空变化规律及局麻预处理对Fos表达的影响。在麻醉下切开足底前，分别注射布比卡因（0.5%）、利多卡因（2%）或生理盐水作为对照，进行神经阻滞和局部浸润。在切口后1、3、6、24、48、72和120 h评估脊髓L4-L5节段的疼痛行为和Fos表达。机械刺激的戒断阈值在1 ~ 120 h （1 ~ 72 h, P < 0.01;120 h, P < 0.05）显著降低，局麻药预处理在1 h（两组：P < 0.01）、3 h（两组：P < 0.01）、6 h（布比卡因，P < 0.01；利多卡因，P < 0.05）较生理盐水组显著升高。在生理盐水组中，Fos主要表达于I-II和V-VI层，总Fos表达量在1 h时达到最大值，随后逐渐降低。局麻预处理显著抑制各层Fos表达，且抑制持续数天。本研究提供了足底切开诱导Fos表达时空变化的证据。我们的研究结果表明，尽管局麻药预处理在术后数天内抑制Fos表达，但仅在使用局麻药的预期时间内观察到镇痛效果。
We investigated the spatial and temporal patterns of c-Fos protein (Fos) expression in the dorsal horn of the spinal cord caused by plantar incision in the rat and the effects of pretreatment with local anesthetics. Bupivacaine (0.5%), lidocaine (2%), or saline for control was injected for nerve block and local infiltration before the plantar incision was made under anesthesia. Pain behavior and Fos expression in the L4-L5 segments of the spinal cord were assessed at 1, 3, 6, 24, 48, 72, and 120 h after the incision. The withdrawal threshold to mechanical stimulation decreased significantly at 1 h until 120 h (1–72 h, P < 0.01;120 h, P < 0.05), and pretreatment with local anesthetics increased the threshold significantly at 1 h (both groups:P < 0.01), 3 h (both groups:P < 0.01), and 6 h (bupivacaine, P < 0.01; lidocaine, P < 0.05) in comparison with that in the saline group. In the saline group, Fos expression was detected predominantly in laminae I–II and V–VI, and the total Fos expression was maximal at 1 h and then decreased gradually. Pretreatment with local anesthetics suppressed Fos expression significantly in all layers, and this suppression continued for several days. This study provides evidence of spatial and temporal changes in Fos expression induced by plantar incision. Our results indicate that although pretreatment with local anesthetics suppresses Fos expression for several days in the postoperative period, the analgesic effect is observed only for the expected duration of the local anesthetic used.