DNA PROBE-MEDIATED DETECTION OF RESISTANT-BACTERIA FROM SOILS HIGHLY POLLUTED BY HEAVY-METALS
DNA PROBE-MEDIATED DETECTION OF RESISTANT-BACTERIA FROM SOILS HIGHLY POLLUTED BY HEAVY-METALS
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DOI：
10.1128/aem.56.5.1485-1491.1990
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发表时间：
1990-05-01
影响因子：
4.4
通讯作者：
MERGEAY, M
中科院分区：
文献类型：
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作者：
DIELS, L;MERGEAY, M
真养产碱杆菌CH 34 DNA片段编码抗CD 2+，Co 2+，Zn 2+（czc），或Hg 2+（merA）克隆和作为探针的菌落杂交程序与细菌分离的污染环境，如锌工厂区（沙漠化，因为锌污染的毒性作用）和沉积物从有色金属冶金厂在比利时和扎伊尔矿区。从不同的土壤样品中，可以分离菌株，并与czc探针（来自质粒pMOL 30的Cd 2+、Co 2+和Zn 2+抗性）杂交。在与czc和抗汞探针杂交的非选择性平板上分离的CFU的回收率，锌沙漠分别为25%和0%，两个比利时工厂分别为15 - 20%和10 - 20%，利卡西矿区分别为40%和40%。这些菌株中的大多数还携带两个与A.真养型菌株CH 34的许多表型性状与该菌株相似。这些结果表明，污染土壤中重金属含量与抗重金属巨质粒A的存在有一定的相关性。真养型菌株
Alcaligenes eutrophus CH34 DNA fragments encoding resistance to CD2+, Co2+, Zn2+ (czc), or Hg2+ (merA) were cloned and used as probes in colony hybridization procedures with bacteria isolated from polluted environments such as a zinc factory area (desertified because of the toxic effects of zinc contamination) and from sediments from factories of nonferrous metallurgy in Belgium and mine areas in Zaire. From the different soil samples, strains could be isolated and hybridized with the czc probe (resistance to Cd2+, Co2+, and Zn2+ from plasmid pMOL30). Percentages of CFU isolated on nonselective plates which hybridized with the czc and the mercury resistance probes were, respectively, 25 and 0% for the zinc desert, 15 to 20 and 10 to 20% for the two Belgian factories, and 40 and 40% for the Likasi mine area. Most of these strains also carried two large plasmids of about the same size as those of A. eutrophus CH34 and shared many phenotypic traits with this strain. These findings indicated a certain correlation between the heavy-metal content in contaminated soils and the presence of heavy-metal-resistant megaplasmid-bearing A. eutrophus strains.