Evaluation of solute permeation through the stratum corneum: Lateral bilayer diffusion as the primary transport mechanism
Evaluation of solute permeation through the stratum corneum: Lateral bilayer diffusion as the primary transport mechanism
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DOI：
10.1021/js960198e
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发表时间：
1997-10-01
影响因子：
3.8
通讯作者：
Langer, R
中科院分区：
文献类型：
--
作者：
Johnson, ME;Blankschtein, D;Langer, R
溶质渗透通过人角质层（SC）的基本双层运输性能方面进行了检查。建立了一个数学模型，用于描述宏观SC通过角质形成细胞间脂质结构域的渗透：（i）SC的结构和尺寸;（ii）微观脂质双层传输特性，包括双层/水分配系数、横向扩散系数、界面跨双层传质系数和膜内跨双层传质系数。的相对重要性与双层传输性能的扩散阻力进行了评估与模型和实验数据。横向扩散系数在SC脂质双层计算120人皮肤渗透性测量，并与以前报道的测量在SC提取的脂质。良好的定性和定量的协议进行了观察，表明，在该模型的背景下，与横向扩散的扩散阻力是足以解释溶质渗透通过SC的整体阻力。类似的分析表明，与界面transbilayer运输的扩散阻力是不能解释的实验渗透值，从而支持这一发现。横向扩散分析还揭示了SC内运输的双功能尺寸依赖性，对于小溶质具有强烈的尺寸依赖性（
Solute permeation across human stratum corneum (SC) was examined in terms of the fundamental bilayer transport properties. A mathematical model was developed to describe the macroscopic SC permeation via the interkeratinocyte lipid domain in terms of (i) the structure and dimensions of the SC, and (ii) the microscale lipid bilayer transport properties, which include the bilayer/water partition coefficient, the lateral diffusion coefficient, the interfacial transbilayer mass transfer coefficient, and the intramembrane transbilayer mass transfer coefficient. The relative importance of the diffusive resistances associated with the bilayer transport properties was evaluated with the model and experimental data. Lateral diffusion coefficients in SC lipid bilayers were calculated from 120 human skin permeability measurements, and compared with previously reported measurements made in SC-extracted lipids. Good qualitative and quantitative agreement was observed, indicating that, in the context of the model, the diffusive resistance associated with lateral diffusion is sufficient to explain the overall resistance of solute permeation through the SC. A similar analysis shows that the diffusive resistance associated with interfacial transbilayer transport is not capable of explaining the experimental permeation values, thus supporting this finding. The lateral diffusion analysis also revealed a bifunctional size dependence of transport within the SC, with a strong size dependence for small solutes (