Molecular imaging of proteins in tissues by mass spectrometry
Molecular imaging of proteins in tissues by mass spectrometry
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DOI：
10.1073/pnas.0801374105
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发表时间：
2008-11-25
影响因子：
11.1
通讯作者：
Caprioli, Richard M.
中科院分区：
文献类型：
--
作者：
Seeley, Erin H.;Caprioli, Richard M.
成像MS（IMS）是一种新兴技术，其允许直接分析和确定组织切片中分子的分布。生物分子如蛋白质、肽、脂质、外源性物质和代谢物可以以高通量方式进行分析，其分子特异性通过其他手段不容易实现。完整地分析组织，因此保留了组织内分子的空间定位。提出了几项研究，重点关注IMS可获得的独特类型的信息，例如阿尔茨海默病斑块中的A β亚型分布、小鼠大脑中的蛋白质图谱以及人类乳腺癌中的空间蛋白质分布。对100年前从淀粉样变性病患者身上取下的活组织检查的分析说明了IMS与福尔马林固定组织的使用。最后，IMS与MRI的配准和相关性。
Imaging MS (IMS) is an emerging technology that permits the direct analysis and determination of the distribution of molecules in tissue sections. Biological molecules such as proteins, peptides, lipids, xenobiotics, and metabolites can be analyzed in a high-throughput manner with molecular specificity not readily achievable through other means. Tissues are analyzed intact and thus spatial localization of molecules within a tissue is preserved. Several studies are presented that focus on the unique types of information obtainable by IMS, such as A beta isoform distributions in Alzheimer's plaques, protein maps in mouse brain, and spatial protein distributions in human breast carcinoma. The analysis of a biopsy taken 100 years ago from a patient with amyloidosis illustrates the use of IMS with formalin-fixed tissues. Finally, the registration and correlation of IMS with MRI is presented.