FUNCTIONAL DISSECTION OF ESCHERICHIA-COLI PROMOTERS - INFORMATION IN THE TRANSCRIBED REGION IS INVOLVED IN LATE STEPS OF THE OVERALL PROCESS
FUNCTIONAL DISSECTION OF ESCHERICHIA-COLI PROMOTERS - INFORMATION IN THE TRANSCRIBED REGION IS INVOLVED IN LATE STEPS OF THE OVERALL PROCESS
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DOI：
10.1002/j.1460-2075.1986.tb04597.x
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发表时间：
1986-11-01
期刊：
影响因子：
11.4
通讯作者：
BUJARD, H
中科院分区：
文献类型：
--
作者：
KAMMERER, W;DEUSCHLE, U;BUJARD, H
在与启动子结合后，大肠杆菌RNA聚合酶与约70bp的区域接触。这个序列大约有20bp被转录。在这个转录区域内编码的信息参与了启动子功能程序的后期步骤。通过改变这些“下游”序列，体内启动子的强度可以变化10倍以上。相比之下，启动子程序的早期步骤的信息，如酶的识别和稳定复合物的形成，位于大约35bp的中央核心区域。我们的数据表明，启动子的强度可以在整个过程的不同水平上受到限制。因此，相同强度的启动子由于其程序的交替优化而表现出不同的结构。
After binding to a promoter Escherichia coli RNA polymerase is in contact with a region of about 70 bp. Around 20 bp of this sequence are transcribed. Information encoded within this transcribed region is involved in late steps of the functional program of a promoter. By changing such ''downstream'' sequences promoter strength in vivo can be varied more than 10-fold. By contrast, information for early steps of the promoter program such as recognition by the enzyme and formation of a stable complex resides in a central core region of about 35 bp. Our data show that the strength of a promoter can be limited at different levels of the overall process. Consequently prmoters of identical strength can exhibit different structures due to an alternate optimization of their program.