Modulation of paired-pulse responses in the dentate gyrus: Effects of normal maturation and vigilance state
Modulation of paired-pulse responses in the dentate gyrus: Effects of normal maturation and vigilance state
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DOI：
10.1114/1.261
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发表时间：
2000-01-01
影响因子：
3.8
通讯作者：
Bronzino, JD
中科院分区：
文献类型：
--
作者：
Blaise, JH;Bronzino, JD
本研究探讨了正常发育和警觉状态对15、30和90日龄自由活动大鼠齿状颗粒细胞活性调制的影响，包括三种警觉状态：安静清醒、慢波睡眠和快速眼动睡眠。采用配对脉冲刺激，获得了由内侧穿支通路刺激引起的齿状回诱发保持电位的配对脉冲指数（PPI），尽管在发育过程中观察到PPI值的显著差异，但没有获得与警觉状态相关的显著变化。断奶前的幼鼠，即，15-与90日龄的成年大鼠相比，在所有三种警戒状态下，30日龄的成年大鼠表现出明显更少的早期（脉冲间隔，IPI= 20-50 ms）和晚期（IPI = 300-1000 ms）抑制，以及更少的易化（CPI = 50-150 ms）。从30日龄组获得的PPI值介于15日龄和90日龄动物之间。PPI值的这些变化提供了在正常成熟过程中齿状颗粒细胞兴奋性的调制变化的定量测量。它们现在可以用于评估各种损伤的影响，如产前蛋白质营养不良或新生儿应激对海马发育的影响。(C)2000生物医学工程学会。【S0090-6964（00）00601-9】。
This study examined the effect of normal development and vigilance state on the modulation of dentate granule cell activity in the freely moving rat at 15, 30, and 90 days of age across three vigilance states: quiet waking, slow-wave sleep, and rapid eye movement sleep. Using paired-pulse stimulation, the paired-pulse index (PPI) was obtained for the dentate evoked held potentials elicited by the stimulation of the medial perforant path. Although significant differences in PPI values were observed during development, no significant vigilance state related changes were obtained. Preweaning infant rats, i.e., 15-day old, exhibited significantly less early (interpulse intervals, IPI= 20-50 ms) and late (IPI = 300-1000 ms) inhibition, and less facilitation (CPI = 50-150 ms) when compared to the 90-day old adult rats during all three vigilance states. PPI values obtained from the 30-day old group fell intermediate between the 15- and 90-day old animals. These changes in PPI values provide a quantitative measure of changes in the modulation of dentate granule cell excitability during normal maturation. They can now can be used to evaluate the impact of various insults, such as prenatal protein malnutrition or neonatal stress, on hippocampal development. (C) 2000 Biomedical Engineering Society. [S0090-6964(00)00601-9].