Changing DNA grooves -: A 1,4,5,8-naphthalene tetracarboxylic dilmide bis-intercalator with the linker (β-ala)3-lys in the minor groove
Changing DNA grooves -: A 1,4,5,8-naphthalene tetracarboxylic dilmide bis-intercalator with the linker (β-ala)3-lys in the minor groove
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DOI：
10.1021/ja016834e
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发表时间：
2002-03-27
影响因子：
15
通讯作者：
Iverson, BL
中科院分区：
文献类型：
--
作者：
Guelev, V;Sorey, S;Iverson, BL
我们一直在研究一个模块化的，线程DNA polyintercalator设计的基础上，1，4，5，8-萘四羧酸二酰亚胺（NDI）嵌入单元。先前，我们已经报道了双嵌入剂-DNA复合物的NMR分析，其中发现NDI单元之间的肽接头占据DNA大沟（Guelev，Lee，Sorey，霍夫曼，Iverson，Chem.Biol.2001，8，415 - 425）。在这里，我们描述了一个相关的双嵌入剂之间的复合物的NMR分析已知显示改变DNA序列特异性。在这种情况下，接头位于DNA小沟中。因此，我们已经表明，在这组序列特异性双嵌入剂中，两个DNA凹槽都可以进入，为设计成具有以交替方式占据两个凹槽的接头的更长的穿线聚嵌入剂设置了阶段。
We have been investigating a modular, threading DNA polyintercalator design based upon the 1,4,5,8-naphthalene tetracarboxylic diimide (NDI) intercalating unit. Previously, we have reported the NMR analysis of a bis-intercalator-DNA complex in which the peptide linker between NDI units was found to occupy the DNA major groove (Guelev, Lee, Sorey, Hoffman, Iverson,Chem. Biol.2001,8, 415−425). Here we describe the NMR analysis of a complex between a related bis-intercalator known to display altered DNA sequence specificity. In this case, the linker resides in the DNA minor groove. We have thus shown that within this set of sequence specific bis-intercalators, both DNA grooves can be accessed, setting the stage for longer threading polyintercalators designed to have linkers occupying both grooves in an alternating fashion.