New Medium for Rapid Quantification of Micrococcus Luteus Cells by Multichannel Series Piezoelectric Quartz Crystal Based Method
New Medium for Rapid Quantification of Micrococcus Luteus Cells by Multichannel Series Piezoelectric Quartz Crystal Based Method
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基于多通道系列压电石英晶体的方法快速定量藤黄微球菌细胞的新培养基
DOI：
10.1166/sl.2010.1318
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发表时间：
2010-08
期刊：
影响因子：
--
通讯作者：
He, Fengjiao
中科院分区：
文献类型：
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作者：
Tu, Jinrong;He, Fengjiao
藤黄微球菌的致病性在免疫功能低下的患者中变得越来越突出。当这种细菌达到一定数量时，即使是健康人也会导致疾病。但迄今为止，关于如何定量藤黄微球菌活细胞的报道很少。本文提出了一种新开发的培养基，用于通过多通道串联压电石英晶体（MSPQC）传感器快速定量藤黄微球菌细胞。使用棉子糖作为碳源来替代新培养基中的葡萄糖。即使使用 0.5% (w/v) 的棉子糖，也可以获得良好的 MSPQC 响应信号。在每毫升 10-107 个菌落形成单位 (cfu) 的范围内，可以通过所提出的方法估计藤黄微球菌 ATCC 4698。采用该方法对59株临床分离的藤黄微球菌进行了评价，准确率约为93.2%。与传统方法相比，该方法简单、快速、易于操作。
The pathogenicity of Micrococcus luteus has become increasingly prominent in immunocompromised patients. This bacterium can cause illness even in healthy people when it reaches the certain number. But so far few reports have been published about how to quantify the viable cells of Micrococcus luteus. In this paper, a new developed culture medium was proposed for rapid quantification of Micrococcus luteus cells by multichannel series piezoelectric quartz crystal (MSPQC) sensor. Raffinose was used as a carbon source to substitute glucose in the new medium. Good response signal of MSPQC can be obtained even if 0.5% (w/v) of raffinose was used. In the range of 10–107 colony forming units (cfu) per ml, Micrococcus luteus ATCC 4698 can be estimated by proposed method. A total of 59 clinical Micrococcus luteus isolates were also evaluated by proposed method and the accuracy rate was about 93.2%. Comparing with conventional method, the proposed method is simple, rapid and easy to operate.