Sulfated modification can enhance the adjuvanticity of lentinan and improve the immune effect of ND vaccine
Sulfated modification can enhance the adjuvanticity of lentinan and improve the immune effect of ND vaccine
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硫酸化修饰可增强香菇多糖的佐剂作用，提高ND疫苗的免疫效果
DOI：
10.1016/j.vaccine.2008.11.038
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发表时间：
2009-01-29
期刊：
影响因子：
5.5
通讯作者：
Liu, Ping
中科院分区：
文献类型：
--
作者：
Guo, Zhenhuan;Hu, Yuanliang;Liu, Ping
研究香菇多糖硫酸酯（sulfated lentinan，sLNT）对纽卡斯尔疫苗免疫效果的影响。免疫应答试验中，14日龄雏鸡接种ND疫苗后，分组分别注射sLNTs，每次2剂，每天1次，连续3 d，以非硫酸化香菇多糖（non-sulfated lentinan，LNT）为对照。检测免疫前后血清抗体效价和外周血淋巴细胞增殖反应的变化。在免疫保护试验中，35日龄雏鸡经上述处理后，用新城疫病毒（NDV）攻毒。观察攻毒前后鸡的发病率和死亡率，测定攻毒前后血清抗体效价和外周血淋巴细胞增殖的变化。结果表明：3种修饰的sLNTs均能显著提高2只试验鸡的血清抗体效价和促进淋巴细胞增殖，降低新城疫攻毒雏鸡的发病率和死亡率，其效果均优于未修饰的UNIT，且以高剂量的sLNTs提高抗体效价和低剂量的sLNTs促进淋巴细胞增殖效果较好。说明硫酸化修饰可增强LNT的佐剂性，提高ND疫苗的免疫效果。其中sLNT（2）的免疫效果最好，有望成为新型免疫佐剂的候选者。(C)2008爱思唯尔有限公司保留所有权利。
The purpose of this research is to Study the effect of sulfated lentinan (sLNT) on immune effect of Newcastle disease (ND) vaccine. In immune response test, 14-day-old chickens were vaccinated with ND vaccine then grouped respectively to inject three sLNTs at two doses, once a day for three successive days, taking non-sulfated lentinan (LNT) as the control. The changes of serum antibody titer and peripheral lymphocyte proliferation were determined before and after vaccination. In immune protection test, 35-day-old chickens were challenged with ND virus (NDV) after treated similar to above mentioned. The morbidity and mortality of chickens were observed, and the changes of serum antibody titer and peripheral lymphocyte proliferation were measured before and after challenge. The results showed that three modified sLNTs; could significantly enhance serum antibody titer and promote lymphocyte proliferation in two experimental chickens, and reduce morbidity and mortality of chickens challenged with NDV, which were better than that of non-modified UNIT Their high doses in enhancing antibody titer and low doses in promoting lymphocyte proliferation were more preferable. These results indicated that sulfated modification could enhance the adjuvanticity of LNT and improve the immune effect of ND vaccine. sLNT(2) possessed the best efficacy and would be expected as the candidate of a new-type immune adjuvant. (C) 2008 Elsevier Ltd. All rights reserved.