Lateral Leaflet Suppression 1 (LLS1), encoding the MtYUCCA1 protein, regulates lateral leaflet development in Medicago truncatula
Lateral Leaflet Suppression 1 (LLS1), encoding the MtYUCCA1 protein, regulates lateral leaflet development in Medicago truncatula
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侧叶抑制 1 (LLS1) 编码 MtYUCCA1 蛋白，调节蒺藜苜蓿侧叶发育
DOI：
10.1111/nph.16539
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发表时间：
2020
期刊：
影响因子：
9.4
通讯作者：
Chen J.
中科院分区：
文献类型：
--
作者：
Zhao B;He L;Jiang C;Liu Y;He H;Bai Q;Zhou S;Zheng X;Wen J;Mysore K. S;Tadege M;Liu Y;Liu R;Chen J.
在具有复叶的物种中，小叶原基起始的位置与生长素积累的局部峰值相关。然而，生长素在发育后期和复叶生长过程中的作用仍然很大程度上未知。利用基因组重测序方法，我们在蒺藜苜蓿编码生长素生物合成酶YUCCA1的侧叶抑制1（LLS1）基因的四个等位基因处鉴定了插入位点。连锁分析和互补测试表明， 这些突变表型是由 Tnt1 插入破坏了 LLS1 基因引起的。 LLS1的转录本可以在叶起始早期的原基中检测到，随后在小叶的基部区域中检测到，最后在叶发育后期的静脉组织中检测到。 ells1-1 突变体中的静脉数量和生长素含量减少。对lls1 sgl1和lls1 palm1双突变体的分析表明，SGL1对LLS1具有上位性，LLS1与PALM1以独立的途径共同调节侧叶的生长。我们的工作表明，YUCCA1/YUCCA4亚群在木犀草复叶发育过程中对侧叶的生长起着非常重要的作用。 truncatula，除了叶脉。
In species with compound leaves, the positions of leaflet primordium initiation are associated with local peaks of auxin accumulation. However, the role of auxin during the late developmental stages and outgrowth of compound leaves remains largely unknown.Using genome resequencing approaches, we identified insertion sites at four alleles of theLATERAL LEAFLET SUPPRESSION1(LLS1) gene, encoding the auxin biosynthetic enzyme YUCCA1 inMedicago truncatula.Linkage analysis and complementation tests showed that thells1mutant phenotypes were caused by theTnt1insertions that disrupted theLLS1gene. The transcripts ofLLS1can be detected in primordia at early stages of leaf initiation and later in the basal regions of leaflets, and finally in vein tissues at late leaf developmental stages. Vein numbers and auxin content are reduced in thells1‐1mutant. Analysis of thells1 sgl1andlls1 palm1double mutants revealed thatSGL1is epistatic toLLS1, andLLS1works withPALM1in an independent pathway to regulate the growth of lateral leaflets.Our work demonstrates that the YUCCA1/YUCCA4 subgroup plays very important roles in the outgrowth of lateral leaflets during compound leaf development ofM. truncatula, in addition to leaf venation.