Heat shock cognate protein 70 is involved in rotavirus cell entry
Heat shock cognate protein 70 is involved in rotavirus cell entry
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DOI：
10.1128/jvi.76.8.4096-4102.2002
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发表时间：
2002-04-01
影响因子：
5.4
通讯作者：
Arias, CF
中科院分区：
文献类型：
--
作者：
Guerrero, CA;Bouyssounade, D;Arias, CF
在这项工作中，我们确定了热休克同源蛋白 (hsc70) 作为轮状病毒的候选受体。 hsc70 被证明存在于 MA104 细胞表面，该蛋白的抗体可阻断轮状病毒的感染性，同时不影响呼肠孤病毒和脊髓灰质炎病毒的感染性。 hsc70 蛋白与病毒预孵育也抑制了病毒的感染性。在固相测定中，三层颗粒（成熟病毒粒子）而非双层颗粒与 hsc70 结合，并且这种相互作用被病毒表面蛋白 VP4 和 VP7 的单克隆抗体阻断。轮状病毒在附着后步骤中与 hsc70 相互作用，因为 hsc70 抗体和蛋白质本身不会抑制病毒附着到细胞上。我们认为功能性轮状病毒受体是几种细胞表面分子的复合体，其中包括 hsc70。
In this work, we have identified the heat shock cognate protein (hsc70) as a receptor candidate for rotaviruses. hsc70 was shown to be present on the surface of MA104 cells, and antibodies to this protein blocked rotavirus infectivity, while not affecting the infectivity of reovirus and poliovirus. Preincubation of the hsc70 protein with the viruses also inhibited their infectivity. Triple-layered particles (mature virions), but not double-layered particles, bound hsc70 in a solid-phase assay, and this interaction was blocked by monoclonal antibodies to the virus surface proteins VP4 and VP7. Rotaviruses were shown to interact with hsc70 at a postattachment step, since antibodies to hsc70 and the protein itself did not inhibit the virus attachment to cells. We propose that the functional rotavirus receptor is a complex of several cell surface molecules that include, among others, hsc70.