Shotgun proteomic analysis of human-induced sputum
Shotgun proteomic analysis of human-induced sputum
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DOI：
10.1002/pmic.200600011
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发表时间：
2006-08-01
期刊：
影响因子：
3.4
通讯作者：
Djukanovic, Ratko
中科院分区：
文献类型：
--
作者：
Nicholas, Ben;Skipp, Paul;Djukanovic, Ratko
诱导痰是一种容易获得的生物液体，其成分可能因疾病而改变。然而，到目前为止，通常填充这种生物流体的蛋白质在很大程度上是未知的，部分原因是处理这种富含粘蛋白的样品的技术困难。为了提供一个痰蛋白的目录，我们调查了人诱导痰的蛋白质组。2-D凝胶分析和GeLC-MS/MS的组合允许总共191种人类蛋白质被确信地分配。除了预期的成分外，还发现了几种迄今未报道的蛋白质，包括膜联蛋白家族的三个成员，激肽释放酶1和11，以及过氧化物酶1，2和5。其他几组蛋白质的鉴定包括四个蛋白质以前注释为假设或保守的假设。总之，这些数据代表了诱导痰蛋白质组的首次广泛调查，并为未来识别肺部疾病的生物标志物提供了平台。
Induced sputum is a readily accessible biological fluid whose composition may alter as a consequence of disease. To date, however, the proteins that routinely populate this biofluid are largely unknown, in part due to the technical difficulties in processing such mucin-rich samples. To provide a catalogue of sputum proteins, we have surveyed the proteome of human-induced sputum (sputome). A combination of 2-D gel analysis and GeLC-MS/MS allowed a total of 191 human proteins to be confidently assigned. In addition to the expected components, several hitherto unreported proteins were found to be present, including three members of the annexin family, kallikreins 1 and 11, and peroxiredoxins 1, 2 and 5. Other sets of proteins identified included four proteins previously annotated as hypothetical or conserved hypothetical. Taken together, these data represent the first extensive survey of the proteome of induced sputum and provide a platform for future identification of biomarkers of lung disease.