Pseudomonas moraviensis sp. nov. and Pseudomonas vranovensis sp. nov., soil bacteria isolated on nitroaromatic compounds, and emended description of Pseudomonas asplenii.
Pseudomonas moraviensis sp. nov. and Pseudomonas vranovensis sp. nov., soil bacteria isolated on nitroaromatic compounds, and emended description of Pseudomonas asplenii.
复制标题
摩拉维假单胞菌 sp.
DOI：
10.1099/ijs.0.63988-0
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发表时间：
2006
影响因子：
2.8
通讯作者：
E. Lang
中科院分区：
文献类型：
--
作者：
L. Tvrzová;P. Schumann;C. Spröer;I. Sedláček;Z. Páčová;O. Šedo;Z. Zdráhal;M. Steffen;E. Lang
对从土壤中分离的两株经硝基芳烃选择性富集的革兰氏阴性菌进行了多相分类研究。根据16 S rRNA基因序列分析，发现这两株菌属于假单胞菌属，在γ-变形菌门。菌株1B 4 T与韩国假单胞菌DSM 16610 T（99.5%）和杰氏假单胞菌CCM 4840 T（99.3%）的同源性最高;菌株2B 2 T与铁角假单胞菌DSM 17133 T（98.9%）、褐阴道假单胞菌DSM 7231 T（98.9%）和恶臭假单胞菌DSM 291 T（98.7%）的同源性最高。根据系统发育分析、DNA-DNA杂交和表型特征，包括化学分类学特征，提出了两个新种：模式菌株为1B 4 T（=CCM 7280 T =DSM 16007 T）的莫拉维假单胞菌（Pseudomonasmoraviensissp. nov.）和模式菌株为2B 2 T（=CCM 7279 T =DSM 16006 T）的弗拉诺维假单胞菌（Pseudomonasvranovensis sp. nov.）。根据本研究中获得的额外数据，对铁角蕨的描述进行了修订。
Two strains of Gram-negative bacteria isolated from soil by selective enrichment with nitroaromatics were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA gene sequence analysis, the two strains were found to belong to the genus Pseudomonas, within the Gammaproteobacteria. Strain 1B4T shared the highest sequence similarity with Pseudomonas koreensis DSM 16610T (99.5%) and Pseudomonas jessenii CCM 4840T (99.3%), and strain 2B2T with Pseudomonas asplenii DSM 17133T (98.9%), Pseudomonas fuscovaginae DSM 7231T (98.9%) and Pseudomonas putida DSM 291T (98.7%). On the basis of phylogenetic analysis, DNA-DNA hybridization and phenotype, including chemotaxonomic characteristics, two novel species, Pseudomonas moraviensis sp. nov. with the type strain 1B4T (=CCM 7280T=DSM 16007T) and Pseudomonas vranovensis sp. nov. with the type strain 2B2T (=CCM 7279T=DSM 16006T), are proposed. The description of P. asplenii was emended on the basis of additional data obtained in this study.