CIGARETTE-SMOKING RELATED POLYCYCLIC AROMATIC HYDROCARBON-DNA ADDUCTS IN PERIPHERAL MONONUCLEAR-CELLS
CIGARETTE-SMOKING RELATED POLYCYCLIC AROMATIC HYDROCARBON-DNA ADDUCTS IN PERIPHERAL MONONUCLEAR-CELLS
复制标题
DOI：
10.1093/carcin/13.11.2041
复制
发表时间：
1992-11-01
期刊：
影响因子：
4.7
通讯作者：
PERERA, FP
中科院分区：
文献类型：
--
作者：
SANTELLA, RM;GRINBERGFUNES, RA;PERERA, FP
血液中与吸烟有关的多环芳烃-DNA加合物的研究产生了相互矛盾的结果。为了确定特定的白血球亚群是否是监测香烟烟雾暴露的有用标记物，研究人员从63名重度吸烟者和27名不吸烟者身上采集了血液。用识别苯并[a]芘和结构相关的二环氧化物-DNA加合物的多克隆抗血清竞争酶联免疫吸附试验测定加合物水平。对吸烟者淋巴细胞和单核细胞成分的分析表明，70%的人有可检测到的加合物，平均值为4.38+/-4.29个加合物/10(8)个核苷酸，而不吸烟者的相应数值分别为22%和1.35+/-0.78/10(8)个核苷酸(P<；0.001)。吸烟者血浆可替宁水平(286+/-90µg/l)与非吸烟者(4.4+/-3.3mug/l)相比有显著差异(P<；0.001)。然而，在这些重度吸烟者中，可替宁与自我报告的吸烟史没有相关性。吸烟者的DNA加合物也与每天吸烟、包年数和血浆可替宁没有相关性，这表明DNA加合物的形成存在很大的个体差异。这些数据表明，吸烟者的淋巴细胞和单核细胞比不吸烟者有更高的多环芳烃二环氧化物-DNA加合物水平。
Studies on cigarette smoking related polycyclic aromatic hydrocarbon-DNA adducts in blood have produced conflicting results. To determine whether a subset of specific white blood cells is a useful marker for monitoring exposure to cigarette smoke, blood was obtained from 63 heavy smokers and 27 non-smokers. Adduct levels were determined by competitive enzyme-linked immunosorbent assay with a polyclonal antiserum recognizing benzo[a]pyrene and structurally related diolepoxide-DNA adducts. Analysis of the lymphocyte plus monocyte fraction from smokers indicated 70% had detectable adducts with a mean of 4.38 +/- 4.29 adducts/10(8) nucleotides, while in non-smokers the corresponding values were 22% and 1.35 +/- 0.78/10(8) (P < 0.001). Plasma cotinine levels differed significantly in smokers (286 +/- 90 mug/1) compared to non-smokers (4.4 +/- 3.3 mug/1) (P < 0.001). However, cotinine was not correlated with self-reported smoking history in these heavy smokers. Nor were DNA adducts in smokers correlated with cigarettes per day, pack-years and plasma cotinine, indicating large interindividual variation in DNA adduct formation. These data demonstrate lymphocytes plus monocytes from smokers have elevated levels of polycyclic aromatic hydrocarbon diolepoxide-DNA adduct levels compared to non-smokers.