Polarization of chemoattractant receptor signaling during neutrophil chemotaxis
Polarization of chemoattractant receptor signaling during neutrophil chemotaxis
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DOI：
10.1126/science.287.5455.1037
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发表时间：
2000-02-11
期刊：
影响因子：
56.9
通讯作者：
Bourne, HR
中科院分区：
文献类型：
--
作者：
Servant, G;Weiner, OD;Bourne, HR
形态极性是哺乳动物细胞趋化所必需的。作为导致这种不对称性的细胞内信号的探针，AKT蛋白激酶(或蛋白激酶B)的Pleckstrin同源结构域被绿色荧光蛋白(PHAKT-GFP)标记，在中性粒细胞中表达。当细胞暴露在化学诱导剂中时，PHAKT-GFP被选择性地招募到细胞前沿的膜上，表明内部信号梯度比化学诱导剂的信号梯度要陡峭得多。艰难梭菌毒素B抑制了PHAKT-GFP的转位，表明它需要一个或多个Rho鸟苷三磷酸酶的活性。
Morphologic polarity is necessary for chemotaxis of mammalian cells. As a probe of intracellular signals responsible for this asymmetry, the pleckstrin homology domain of the AKT protein kinase (or protein kinase B), tagged with the green fluorescent protein (PHAKT-GFP), was expressed in neutrophils. Upon exposure of cells to chemoattractant, PHAKT-GFP is recruited selectively to membrane at the cell's leading edge, indicating an internal signaling gradient that is much steeper than that of the chemoattractant. Translocation of PHAKT-GFP is inhibited by toxin-B from Clostridium difficile, indicating that it requires activity of one or more Rho guanosine triphosphatases.