EFFECTS OF NEONATAL SYMPATHECTOMY AND CAPSAICIN TREATMENT ON BONE REMODELING IN RATS
EFFECTS OF NEONATAL SYMPATHECTOMY AND CAPSAICIN TREATMENT ON BONE REMODELING IN RATS
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DOI：
10.1016/0306-4522(91)90094-5
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发表时间：
1991-01-01
期刊：
影响因子：
3.3
通讯作者：
ELDE, R
中科院分区：
文献类型：
--
作者：
HILL, EL;TURNER, R;ELDE, R
骨代谢可能受到骨骼组织神经支配的影响。神经肽如来自交感神经的血管活性肠肽和来自感觉神经的降钙素基因相关肽已被认为是骨代谢的局部调节剂。研究了新生大鼠交感神经切除术和辣椒素诱导的感觉去神经对以下方面的影响：（i）胫骨（正常生长和建模）中的桡骨生长和沉积速率;（ii）诱导重建期间破骨细胞占据下颌骨骨膜表面的百分比。新生大鼠用乙啶、辣椒素或适当的载体处理。在第七周，去除上颌磨牙以诱导下颌骨颊面的重塑。手术后4天处死动物。通过组织形态计量学测量胫骨的皮质面积、髓质面积和骨膜附着率。在冷冻、未脱钙切片中测量破骨细胞（酸性磷酸酶染色）占据的重塑部位骨膜表面的百分比。各药物处理组与对照组相比，骨皮质面积、骨髓质面积及骨膜附着率均无显著性差异。然而，与对照组相比，用乙啶治疗的动物中破骨细胞占据的下颌骨表面增加了45.5%（P小于或等于0.005）。与此相反，下颌骨表面所占的破骨细胞减少了21.2%（P小于或等于0.04），在posteratally与辣椒素治疗的动物相比controls. Alteration的骨重建（破骨细胞表面）由两种治疗表明，感觉和交感神经在骨的局灶性代谢中发挥作用。
Bone metabolism may be influenced by the innervation of skeletal tissues. Neuropeptides such as vasoactive intestinal peptide, from sympathetic nerves, and calcitonin gene-related peptide, from sensory nerves, have been implicated as local modulators of bone metabolism. The effect of neonatal sympathectomy and of capsaicin-induced sensory denervation in rats was studied on the following: (i) the radial bone growth and apposition rate in tibiae (normal growth and modeling) and (ii) the percentage of periosteal surface of the mandible occupied by osteoclasts during induced remodeling. Neonate rats were treated with guanethidine, capsaicin, or appropriate vehicle. At seven weeks, maxillary molars were removed to induce remodeling on the buccal surface of the mandible. Animals were killed four days after surgery. Cross-sectional cortical area, medullary area, and periosteal apposition rate were measured by histomorphometry in ground sections of tibiae. The percentage of periosteal surface at the remodeling site occupied by osteoclasts (stained for acid phosphatase) was measured in frozen, undecalcified sections. There was no significant difference in cortical or medullary area or periosteal apposition rate in tibiae between each drug treatment and its control. However, the mandibular bone surface occupied by osteoclasts was increased 45.5% (P less-than-or-equal-to 0.005) in animals treated neonatally with guanethidine compared to controls. In contrast, the mandibular surface occupied by osteoclasts was decreased 21.2% (P less-than-or-equal-to 0.04) in animals treated neonatally with capsaicin compared to controls.The alteration of bone remodeling (osteoclast surface) by both treatments indicates that sensory and sympathetic nerves play a role in focal metabolism of bone.