A promoterless retroviral vector indicates that there are sequences in U3 required for 3' RNA processing.
A promoterless retroviral vector indicates that there are sequences in U3 required for 3' RNA processing.
复制标题
无启动子逆转录病毒载体表明 U3 中存在 3 RNA 加工所需的序列。
DOI：
10.1073/pnas.84.5.1197
复制
发表时间：
1987
影响因子：
11.1
通讯作者：
Temin,HM
中科院分区：
文献类型：
--
作者：
Dougherty,JP;Temin,HM
我们构建了一个逆转录病毒载体，其中所有的病毒转录信号在前病毒形成过程中都被删除。这种载体在基因转移实验中会更安全、更有用。该载体的构建涉及从右侧长末端重复序列中删除除整合所需的10个碱基对之外的所有U3序列。我们发现，这种缺失导致无法有效地传播病毒。当我们在载体末端插入包含猴病毒40晚期mRNA多聚腺苷化信号的序列时，我们能够有效地繁殖病毒。这一结果表明，在AAUAAA上游的U3中存在对病毒RNA的3‘加工至关重要的序列。
We constructed a retrovirus vector in which all viral transcriptional signals are deleted during provirus formation. This vector would be safer and more useful in gene-transfer experiments. Construction of this vector involved the deletion of all of the U3 sequence except for 10 base pairs, required for integration, from the right-side long terminal repeat. We found that this deletion resulted in an inability to propagate virus efficiently. When we inserted sequences containing all of the signals for polyadenylylation of simian virus 40 late mRNA at the end of our vector, we were able to propagate virus efficiently. This result indicates that there are sequences in U3 upstream from AAUAAA that are essential for 3' processing of viral RNA.